Strain Data Sheet

RBRC09734

Strain Information

Image
BRC No.RBRC09734
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Tg(CAG-EGFP,-IL6/mCherry)29Kenw
Former Common nameLGL-IL6 (Li29)
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearance
Strain developmentDeveloped by Ken Watanabe, National Center for Geriatrics and Gerontology in 2015. C57BL/6N genetic background.
Strain descriptionTransgenic mouse line to express Human IL-6 in a Cre-dependent manner. CAG promoter drives loxP-GFP-pA-loxP-IL-6-2A-mCherry-pA. Another transgenic subline of RBRC09734 is available as RBRC09735.
Colony maintenance
References
Efficient selection for high-expression transfectants with a novel eukaryotic vector.
H Niwa, K Yamamura, J Miyazaki
Gene, 108(2):193-9 (1991). 1660837

Generation of a transgenic mouse line for conditional expression of human IL-6.
Taiki Mori, Yusuke Murasawa, Rina Ikai, Tomoko Hayakawa, Hiroyuki Nakamura, Noboru Ogiso, Shumpei Niida, Ken Watanabe
Exp. Anim., 65(4):455-463 (2016). 27349442

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
GH Growth hormone polyA (Bovine)UNGH
IL6 interleukin 6 (human)UNIL6
T2A Thosea asigna virus 2A peptide (insect)UNT2A
loxP phage P1 loxPUNloxP
loxP phage P1 loxPUNloxP
mCherry Red Fluorescent Protein (Discosoma sp.)UNmCherry
SV40 polyA signalUN
mouse Proacrosin signal sequenceUN
Thy-1 GPI anchor signal sequenceUN

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • normal phenotype (MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Bacteriophage loxP sites, Aequorea victoria EGFP cDNA, SV40 PolyA signal, Human IL6 cDNA, Thosea asigna virus 2A peptide (T2A), Mouse Proacrosin signal sequence, Discosoma sp. mCherry cDNA, Thy-1 GPI anchor signal sequence, Bovine GH polyA signal
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    covid19_onset
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mori et al. Exp. Anim. (2016)
    DepositorKen Watanabe (National Center for Geriatrics and Gerontology)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: TBUSAMTA Nonprofit
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data