RBRC09734

Information1

Image
BRC No.RBRC09734
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Tg(CAG-EGFP,-IL6/mCherry)29Kenw
Former Common nameLGL-IL6 (Li29)
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearance
Strain developmentDeveloped by Ken Watanabe, National Center for Geriatrics and Gerontology in 2015. C57BL/6N genetic background.
Strain descriptionTransgenic mouse line to express Human IL-6 in a Cre-dependent manner. CAG promoter drives loxP-GFP-pA-loxP-IL-6-2A-mCherry-pA. Another transgenic subline of RBRC09734 is available as RBRC09735.
Colony maintenance
ReferencesExp. Anim., (2016). 27349442

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
EGFPEnhanced Green Fluorescent Protein, F64L and S65T (Aequorea victoria)UNEGFPCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHGrowth hormone polyA (Bovine)UNGH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IL6interleukin 6 (human)UNIL6

Gene symbolGene nameChr.Allele symbolAllele namePromoter
T2AThosea asigna virus 2A peptide (insect)UNT2A

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
mCherryRed Fluorescent Protein (Discosoma sp.)UNmCherry

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 PolyA signalUN

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Thy-1 GPI anchor signal sequenceUN

Gene symbolGene nameChr.Allele symbolAllele namePromoter
mouse Proacrosin signal sequenceUN

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Bacteriophage loxP sites, Aequorea victoria EGFP cDNA, SV40 PolyA signal, Human IL6 cDNA, Thosea asigna virus 2A peptide, Mouse Proacrosin signal sequence, Discosoma sp. mCherry cDNA, Thy-1 GPI anchor signal sequence, Bovine GH polyA signal
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mori et al. Exp. Anim. (2016)
DepositorKen Watanabe (National Center for Geriatrics and Gerontology)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Category I MTA: FP license of TBUSA
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data