RBRC09731

Information1

Image
BRC No.RBRC09731
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.Cg-Braf<tm1.1Tumg>
Former Common nameBraf Q241R/+ ICR (Braf Q241R knock-in mice; ICR background)
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Yoko Aoki and Shin-ichi Inoue, Tohoku University School of Medicine in 2014. BRUCE-4 ES cells were used. RBRC09730 was crossed with CAG-Cre and then backcrossed to ICR for 11 generations.
Strain descriptionBraf-mutated protein (BrafQ241R) knock-in mice in ICR genetic background. Q241R mutation in mouse Braf corresponds to Q257R in human BRAF, the most frequent mutation in the cardio-facio-cutaneous (CFC) syndrome. Conditional BrafQ241R knock-in mice are available as RBRC09730.
Colony maintenance
ReferencesHuman Molecular Genetics, 24, 7349-7360 (2015). 26472072
Human Molecular Genetics, 23, 6553-6566 (2014). 25035421

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
BrafBraf transforming gene6Braf<tm1(Q241R)Tumg>targeted mutation 1.1, Yoko Aoki

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Information2

Donor DNABacteriophage P1 loxP site, mouse Braf genomic DNA
Research applicationCancer Research
Cell Biology Research
Cre/loxP system
Mouse Models for Human Disease
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Human Molecular Genetics, 23, 6553-6566 (2014). Human Molecular Genetics, 24, 7349-7360 (2015).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorYoko Aoki (Tohoku University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data