RBRC09729

Information1

Image
BRC No.RBRC09729
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.Cg-Hist1h2aa/Hist1h2ba<tm1Sis>
Former Common nameTh2a- Th2b-
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainBALB/cAJcl
Appearance
Strain developmentDeveloped by Shunsuke Fujii, RIKEN in 2014. R1 ES cell line [(129X1/SvJ x 129S1)F1] was used. BALB/cA congenic line. C57BL/6 background is available at RBRC09728.
Strain descriptionA 3.6 kb region, which covers both Hist1h2aa and Hist1h2ba, was replaced with a neo cassette via homologous recombination. Male homozygotes are sterile. Female homozygotes have low fertility.
Colony maintenance
ReferencesDevelopment, 142, 1287-1292 (2015). 25742800
Cell Stem Cell., 14, 217-227 (2014). 24506885

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Hist1histone cluster 113Hist1<tm1Sis>targeted mutation 1, Shunsuke Ishii

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)13mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse Phosphoglycerate kinase promoter (PGK promoter), E coli Neomycin resistance gene, mouse Phosphoglycerate kinase polyA signal, mouse Hist1h2aa/Hist1h2ba genomic DNA
Research applicationCell Biology Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Stem Cell., 14, 217-227 (2014). Development, 142, 1287-1292 (2015).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from use of the BIOLOGICAL RESOURCE.
DepositorShunsuke Ishii (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data