RBRC09721

Information1

Image
BRC No.RBRC09721
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC3H-Tg(CAG-Il1rl1,CAG-GFP)23Tomi
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Shin-ichi Tominaga and Kenji Kuroiwa, Jichi Medical University. DNA constructs (CAG-Il1rl1 and CAG-GFP) were injected to fertilized eggs derived from C3H/HeJ. Mice were backcrossed to C3H/HeN for 7 generations.
Strain descriptionTransgenic mice expressing GFP and a secreted form of Il1rl1 (interleukin 1 receptor-like 1; alternative name, ST2) under the control of CAG promoter. Hundreds ng/ml of ST2 can be detected in serum of transgenic mice. Related transgenic mouse lines are available as RBRC09720, RBRC09722 and RBRC09723. BALB/c background (RBRC09691) and C3H/HeJ background (RBRC09725) are also available.
Colony maintenance
ReferencesEur. J. Immunol., 40, 2632-2642 (2010). 20662097

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFP(S65T/S147P)Green Fluorescent Protein (Jellyfish)UNGFP(S65T/S147P)CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Il1rl1interleukin 1 receptor-like 1UNIl1rl1CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), mouse Il1rl1 cDNA, jellyfish GFP(S65T/S147P) cDNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Eur. J. Immunol., 40, 2632-2642 (2010).Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the Dr. Tsukasa Ohmori, Jichi Medical University using the Approval Form. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorShin-ichi Tominaga (Jichi Medical University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data