Strain Data Sheet

RBRC09714

Strain Information

Image
BRC No.RBRC09714
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6(C3)-Vsx2<tm1.1(cre/ERT2)Tfur>
Former Common nameChx10-IRES-CreERT2 KI
H-2 Haplotype
ES Cell lineJM8A3 [C57BL/6N-A<tm1Brd>]
Background strain
Appearance
Strain developmentDeveloped by Takahisa Furukawa, Institute for Protein Research, Osaka University. JM8A3 (C57BL/6N) ES cell line was used. This knock-in mice were mated to mice in B6.C3H background and then mated to mice in C57BL/6J background.
Strain descriptionIRES-CreERT2 knock-in mice. An IRES-CreERT2 cassette was placed just downstream of stop codon of the Vsx2 (visual system homeobox 2, Chx10) gene. A Neo gene cassette was removed by a CAG-Cre mouse line. IRES-CreERT2 can be removed by a Flp recombinase.
Colony maintenanceHeterozygote x Wild-type [or Crossing to C57BL/6JJmsSlc]
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
ERT2 estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)12ERT2
Frt yeast FRT (flippase recombination target) site12Frt
Frt yeast FRT (flippase recombination target) site12Frt
Vsx2
MGI:88401		visual system homeobox 212Vsx2<tm1.1(cre/ERT2)Tfur> targeted mutation 1.1, Takahisa Furukawa
  • isolated microphthalmia 2(MedGEN)

  • microphthalmia, isolated, with coloboma 3(MedGEN)
    • cre Phage P1 Cre recombinase12cre
    loxP phage P1 loxP12loxP

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • obsolete no phenotypic analysis (MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAyeast FRT (flipase recombination target) sites, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), phage P1 Cre, Human ERT2 (human mutant-type mouse estrogen receptor)cDNA, phage P1 loxP site, mouse Vsx2 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Cre driver
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).　The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must perform the study on a collaboration basis with the DEPOSITOR.
    DepositorTakahisa Furukawa (Osaka University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Cre-ERT(2): NOTICE TO RECIPIENT
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data