RBRC09714

Information1

Image
BRC No.RBRC09714
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6(C3)-Vsx2<tm1.1(cre/ERT2)Tfur>
Former Common nameChx10-IRES-CreERT2 KI
H-2 Haplotype
ES Cell lineJM8A3 [C57BL/6N-A<tm1Brd>]
Background strain
Appearance
Strain developmentDeveloped by Takahisa Furukawa, Institute for Protein Research, Osaka University. JM8A3 (C57BL/6N) ES cell line was used. This knock-in mice were mated to mice in B6.C3H background and then mated to mice in C57BL/6J background.
Strain descriptionIRES-CreERT2 knock-in mice. An IRES-CreERT2 cassette was placed just downstream of stop codon of the Vsx2 (visual system homeobox 2, Chx10) gene. A Neo gene cassette was removed by a CAG-Cre mouse line. IRES-CreERT2 can be removed by a Flp recombinase.
Colony maintenanceHeterozygote x Wild-type [or Crossing to C57BL/6JJmsSlc]
References

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Vsx2visual system homeobox 212Vsx2<tm1.1(cre/ERT2)Tfur>targeted mutation 1.1, Takahisa Furukawa

Gene symbolGene nameChr.Allele symbolAllele namePromoter
ERT2estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)12ERT2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site12Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site12Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
crePhage P1 Cre recombinase12cre

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP12loxP

Information2

Donor DNAyeast FRT (flipase recombination target) sites, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), phage P1 Cre, Human ERT2 (human mutant-type mouse estrogen receptor)cDNA, phage P1 loxP site, mouse Vsx2 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must perform the study on a collaboration basis with the DEPOSITOR.
DepositorTakahisa Furukawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data