Strain Data Sheet
RBRC09713
Information1 | |
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| Image | |
| BRC No. | RBRC09713 |
| Type | Targeted Mutation and Transgene |
| Species | Mus musculus |
| Strain name | ICR.Cg-Gata3<tm1.1(GFP)Mpu>Tg(Gata3)1Mpu |
| Former Common name | G3YR mouse |
| H-2 Haplotype | |
| ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Takashi Moriguchi, Tohoku Medical and Pharmaceutical University. R1 ES cell line [(129X1/SvJ x 129S1/Sv)F1] was used. ICR background. |
| Strain description | Bigenic mouse strain generated by crossing two strains, GATA-3 knockdown [Gata3<tm1.1(GFP)Mpu>] and GATA3 YAC transgene [Tg(Gata3)1Mpu]. Homozygous GATA-3 knockdown mice are neonatally lethal due to the cleft palate, but can be rescued by GATA3 YAC transgene. This Gata3 hypomorphic mutant mice rescued with the YAC transgene exhibit a glomerular mesangial cell defect. |
| Colony maintenance | |
| References | Mol. Cell Biol., 36(17):2272-2281 (2016). 27296697 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter DsRed2Red Fluorescent Protein (Discosoma sp.)2DsRed2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site2Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GFPGreen Fluorescent Protein (Aequorea victoria)2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP2loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP2loxP |
Information2 | |
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| Donor DNA | Phage P1 loxP sites, mouse Gata3 cDNA, Jellyfish GFP cDNA (Green Fluorescence Protein), yeast FRT (flipase recombination target) site, Discosoma sp. DsRed2 cDNA(Discosoma sp. Red Fluorescent Protein), mouse Gata3 genomic DNA |
| Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell Biol., 36(17):2272-2281 (2016).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | Takashi Moriguchi (Tohoku Pharmaceutical University) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Genotyping protocol -PCR- Necessary documents for ordering: |
BRC mice in Publications |
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