Strain Data Sheet

RBRC09713

Information1

Image
BRC No.RBRC09713
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameICR.Cg-Gata3<tm1.1(GFP)Mpu>Tg(Gata3)1Mpu
Former Common nameG3YR mouse
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Takashi Moriguchi, Tohoku Medical and Pharmaceutical University. R1 ES cell line [(129X1/SvJ x 129S1/Sv)F1] was used. ICR background.
Strain descriptionBigenic mouse strain generated by crossing two strains, GATA-3 knockdown [Gata3<tm1.1(GFP)Mpu>] and GATA3 YAC transgene [Tg(Gata3)1Mpu]. Homozygous GATA-3 knockdown mice are neonatally lethal due to the cleft palate, but can be rescued by GATA3 YAC transgene. This Gata3 hypomorphic mutant mice rescued with the YAC transgene exhibit a glomerular mesangial cell defect.
Colony maintenance
ReferencesMol. Cell Biol., 36(17):2272-2281 (2016). 27296697

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gata3GATA binding protein 32Gata3<tm1.1(GFP)Mpu>targeted mutation 1.1, Takashi Moriguchi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
DsRed2Red Fluorescent Protein (Discosoma sp.)2DsRed2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site2Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Information2

Donor DNAPhage P1 loxP sites, mouse Gata3 cDNA, Jellyfish GFP cDNA (Green Fluorescence Protein), yeast FRT (flipase recombination target) site, Discosoma sp. DsRed2 cDNA(Discosoma sp. Red Fluorescent Protein), mouse Gata3 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell Biol., 36(17):2272-2281 (2016).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.　The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE
DepositorTakashi Moriguchi (Tohoku Pharmaceutical University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: FP license of TBUSA
  4. Acceptance of responsibility for living modified organism (Japanese / English)
  5. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

Genotyping protocol -PCR-

BRC mice in Publications

No Data