Strain Data Sheet

RBRC09713

Strain Information

Image
BRC No.RBRC09713
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameICR.Cg-Gata3<tm1.1(GFP)Mpu>Tg(Gata3)1Mpu
Former Common nameG3YR mouse
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Takashi Moriguchi, Tohoku Medical and Pharmaceutical University. R1 ES cell line [(129X1/SvJ x 129S1/Sv)F1] was used. ICR background.
Strain descriptionBigenic mouse strain generated by crossing two strains, GATA-3 knockdown [Gata3<tm1.1(GFP)Mpu>] and GATA3 YAC transgene [Tg(Gata3)1Mpu]. Homozygous GATA-3 knockdown mice are neonatally lethal due to the cleft palate, but can be rescued by GATA3 YAC transgene. This Gata3 hypomorphic mutant mice rescued with the YAC transgene exhibit a glomerular mesangial cell defect.
Colony maintenance
References
Gata3 Hypomorphic Mutant Mice Rescued with a Yeast Artificial Chromosome Transgene Suffer a Glomerular Mesangial Cell Defect.
Moriguchi T, Yu L, Otsuki A, Ainoya K, Lim K C, Yamamoto M, Engel J D
Mol. Cell Biol., 36(17):2272-2281 (2016). 27296697

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
DsRed2Red Fluorescent Protein (Discosoma sp.)2DsRed2
  • hypoparathyroidism-deafness-renal disease syndrome(DisGeNET, MedGEN)
    • Frtyeast FRT (flippase recombination target) site2Frt
  • hypoparathyroidism-deafness-renal disease syndrome(DisGeNET, MedGEN)
    • GFPGreen Fluorescent Protein (Aequorea victoria)2
  • hypoparathyroidism-deafness-renal disease syndrome(DisGeNET, MedGEN)
    • Gata3GATA binding protein 32Gata3targeted mutation 1.1, Takashi Moriguchi
  • hypoparathyroidism-deafness-renal disease syndrome(DisGeNET, MedGEN)
    • loxPphage P1 loxP2loxP
  • hypoparathyroidism-deafness-renal disease syndrome(DisGeNET, MedGEN)
    • loxPphage P1 loxP2loxP
  • hypoparathyroidism-deafness-renal disease syndrome(DisGeNET, MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal glomerular mesangium morphology(MP:0011339)

  • abnormal urine creatine level(MP:0030631)

  • decreased nephron number(MP:0011290)

  • kidney cortex atrophy(MP:0011423)

  • kidney failure(MP:0003606)
    • more 3 phenotypes
  • kidney medulla atrophy(MP:0011422)

  • renal fibrosis(MP:0003985)

  • renal tubular necrosis(MP:0004154)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, mouse Gata3 cDNA, Jellyfish GFP cDNA (Green Fluorescence Protein), yeast FRT (flipase recombination target) site, Discosoma sp. DsRed2 cDNA(Discosoma sp. Red Fluorescent Protein), mouse Gata3 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell Biol., 36(17):2272-2281 (2016).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorTakashi Moriguchi (Tohoku Pharmaceutical University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: TBUSAMTA Nonprofit
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data