RBRC09711

Information1

Image
BRC No.RBRC09711
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Tg(Arc-tTA/EGFP/Capzb<*>2)100Mkyph
Former Common nameACE-TG, AiCE-Tg
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrlCrlj
Appearance
Strain developmentDeveloped by Maki Yamada, Tokushima Bunri University, Kagawa School of Pharmaceutical Sciences. A DNA construct was injected to fertilized eggs derived from C57BL/6N. Mice were maintained in C57BL/6N background.
Strain description
Colony maintenance
ReferencesCerebral Cortex,19, 2572-2578 (2009). 19240139
Genes to Cells, 15, 737-747 (2010). 20545768
Sneak Peek (Preprint) doi: 10.2139/ssrn.3419080

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Capzbcapping protein (actin filament) muscle Z-line, betaUNCapzb

Gene symbolGene nameChr.Allele symbolAllele namePromoter
EGFPEnhanced Green Fluorescent Protein, F64L and S65T (Aequorea victoria)UNEGFP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
P2Aporcine teschovirus-1 2A peptideUNP2A

Gene symbolGene nameChr.Allele symbolAllele namePromoter
tTAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)UNtTAmouse Arc promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signalUN

Information2

Donor DNAmouse Arc promoter, tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain; Clontech Tet-Off Advanced Inducible Gene Expression System), Porcine teschovirus 2A cDNA, jellyfish EGFP cDNA from Clontech EGFP-C1 vector, mouse CapZ cDNA, SV40 polyA signal from Clontech EGFP-C1 vector[update 07/14/2020]
Research applicationFluorescent Proteins/lacZ System
Tet system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Fluorescent Marker of a Plasticity-Related Change in Dendritic Spines. Sneak Peek (Preprint) doi: 10.2139/ssrn.3419080For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorMaki Yamada (Tokushima Bunri University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Sequence of AiCE-Transgene

BRC mice in Publications

No Data