Strain Data Sheet

RBRC09702

Strain Information

Image
BRC No.RBRC09702
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Mta1<tm1Nkcc>
Former Common nameMta1 KO
H-2 Haplotype
ES Cell lineE14TG2a [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Soichi Takiguchi, National Hospital Organization Kyushu Cancer Center, in collaboration with Graduate School of Pharmaceutical Sciences, The University of Tokyo in 2002. E14TG2aIV ES cell line (129P2/OlaHsd) was used. C57BL/6J congenic strain (backcrossed to C57BL/6J for 35 generations).
Strain descriptionMta1 (metastasis associated 1) knockout mice. A genomic fragment corresponding from exon 10 to exon 13 of the Mta1 gene was replaced with a Neo resistance gene. The rate of homozygous mutants born from hetero x hetero crossing is much lower than what is expected from the Mendel’s law of inheritance.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Mta1metastasis associated 112Mta1targeted mutation 1, Soichi Takiguchi
neoneomycin resistance gene (E. coli)12mouse RNA polymerase II promoter (PolII promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal bone mineralization(MP:0002896)

  • decreased angiogenesis(MP:0005602)

  • decreased bone ossification(MP:0020040)

  • decreased bone resorption(MP:0004993)

  • decreased compact bone mass(MP:0010962)
    • more 9 phenotypes
  • decreased osteoblast cell number(MP:0004989)

  • decreased osteoclast cell number(MP:0004985)

  • decreased trabecular bone mass(MP:0010873)

  • mortality/aging(MP:0010768)

  • osteoporosis(MP:0000066)

  • small adrenal glands(MP:0002768)

  • small liver(MP:0000601)

  • small ovary(MP:0001127)

  • small testis(MP:0001147)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Polr2a promoter, E. coli neo, human hypoxanthine phosphoribosyl transferase 1 polyA signal, mouse Mta1 genomic DNA
    Research applicationCancer Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use or publication with the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. However, it may not be a collaboration if the research interest is out of field of the DEPOSITOR.
    DepositorSoichi Takiguchi (National Hospital Organization Kyushu Cancer Center)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data