Strain Data Sheet

RBRC09699

Strain Information

Image
BRC No.RBRC09699
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6N-Chd9<em2Ibbr>
Former Common nameB6.Chd9 KO
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearance
Strain developmentDeveloped by Fugaku Aoki, The University of Tokyo in 2016. Fertilized eggs derived from C57BL/6N were used. C57BL/6N background.
Strain descriptionChd9 (chromodomain helicase DNA binding protein 9) mutant mice generated by the Genome editing technique (CRISPR/Cas9). This mutant contains 1 bp deletion within the Chd9 open reading frame causing a frameshift. The mutant mouse is predicted to express a 356 amino acid polypeptide, in contrast to 2869 amino acid native polypeptide. Homozygous mutants are viable and fertile. 8 bp deletion mutant of Chd9 is also available in RBRC09698.
Colony maintenance
References
Chromodomain helicase DNA-binding proteins in stem cells and human developmental diseases.
Micucci J A, Sperry E D, Martin D M
Stem Cells Dev 24(8)917-926 (2015). 25567374

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Chd9chromodomain helicase DNA binding protein 99Chd9endonuclease-mediated mutation 2, Fugaku Aoki

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.)
    DepositorFugaku Aoki (The University of Tokyo)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data