RBRC09690

Information1

Image
BRC No.RBRC09690
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Gpr65<Tn(sb-lacZ,GFP)T1.88Jtak>/Sati
Former Common nameGpr65gt/gt
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Junji Takeda, Osaka University in 2003. Backcrossed to C57BL/6 for 9 generations by Satoshi Ishii, Akita University Graduate School of Medicine.
Strain descriptionGene trap mice of the Gpr65 gene with a sleeping beauty transposable element. Homozygous mice are viable and fertile, but they show abnormal macrophage physiology and osteoclastic activity.
Colony maintenance
ReferencesFASEB J., 28, 871-879 (2014). 24221084
J. Immunol., 182, 3243-3251 (2009). 19234222
J. Neurochem., 129, 683-695 (2014). 24447140
Mol. Cell Biol., 23, 9189-9207 (2003). 14645530

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gpr65G-protein coupled receptor 6512Gpr65<Tn(sb-lacZ,GFP)T1.88Jtak>transposon insertion T1.88, Junji Takeda

Gene symbolGene nameChr.Allele symbolAllele namePromoter
BCL2Splice acceptor (human bcl-2)12BCL2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)12CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Hprtmouse Hprt splice donor (SD)12Hprt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Iresinternal ribosomal entry site (EMCV)12

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)12

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, Phage P1 loxP sites, Salmon Sleeping Beauty transposase, Human bc1-2 splice acceptor (SA), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Simian virus 40 Large T antigen nuclear localization signal (NLS), E. coli lacZ, Rabbit beta-globin adenylation signal sequence, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Jellyfish GFP cDNA, Mouse Hprt splice donor (SD), mRNA instability (human granulocyte-macrophagecolony-stimulating factor cDNA)
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell Biol., 23, 9189-9207 (2003). FASEB J., 28, 871-879 (2014).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorSatoshi Ishii (Akita University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data