RBRC09688

Information1

Image
BRC No.RBRC09688
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.Cg-Siglech<tm1.1Ksat>
Former Common nameSiglechdtr/dtr mice
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strainBALB/cAJcl
Appearance
Strain developmentDeveloped by Katsuaki Sato, RIKEN Research Center for Allergy and Immunology in 2016. Bruce 4 ES cells derived from B6.Cg-Thy1<a> were used. Mice were backcrossed to BALB/c for 12 generations.
Strain descriptionIRES-DTR-EGFP cassette were inserted into 3' untranslated region of Siglech, Siglech sialic acid binding Ig-like lectin H gene, highly expressed in plasmacytoid dendritic cells (pDCs). Homozygous mutant mice are defective in the transcriptional and cell surface expression of Siglech. Mice treated with diphtheria toxin (DT) cause specific ablation of pDCs.
Colony maintenanceHomozygote x Homozygote [or Crossing to BALB/cAJcl]
ReferencesJ. Allergy Clin. Immunol., (2018). 29477579
Immunity, 35, 958-971 (2011). 22177923
Sci. Rep., 6, 24477 (2016). 27075414

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Siglechsialic acid binding Ig-like lectin H7Siglech<tm1.1Ksat>targeted mutation 1.1, Katsuaki Sato

Gene symbolGene nameChr.Allele symbolAllele namePromoter
EGFPEnhanced Green Fluorescent Protein, F64L and S65T (Aequorea victoria)7EGFP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
HBEGFheparin-binding EGF-like growth factor (human)7HBEGF

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Ires2internal ribosomal entry site (EMCV)7Ires2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP7loxP

Information2

Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site2 (ires2), human HB-EGF cDNA, Aequorea Victoria EGFP cDNA, phage P1 loxP site, mouse Siglech genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Immunity, 35, 958-971 (2011). Sci. Rep., 6, 24477 (2016).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR (Dr. Katsuaki Sato). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a “MTA for Siglech-IRES2DTREGFP Knock-In Micefor Academic Research Purposes” specified by the DEPOSITOR and Inserm.
DepositorKatsuaki Sato (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
MTA for Siglech-IRES2DTREGFP Knock-In Mice for Academic Research Purposes (English)
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Depositor's present address; Division of Immunology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki.

BRC mice in Publications

No Data