Strain Data Sheet

RBRC09688

Strain Information

Image
BRC No.RBRC09688
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.Cg-Siglech<tm1.1Ksat>
Former Common nameSiglechdtr/dtr mice
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strainBALB/cAJcl
Appearance
Strain developmentDeveloped by Katsuaki Sato, RIKEN Research Center for Allergy and Immunology in 2016. Bruce 4 ES cells derived from B6.Cg-Thy1<a> were used. Mice were backcrossed to BALB/c for 12 generations.
Strain descriptionIRES-DTR-EGFP cassette were inserted into 3' untranslated region of Siglech, Siglech sialic acid binding Ig-like lectin H gene, highly expressed in plasmacytoid dendritic cells (pDCs). Homozygous mutant mice are defective in the transcriptional and cell surface expression of Siglech. Mice treated with diphtheria toxin (DT) cause specific ablation of pDCs.
Colony maintenanceHomozygote x Homozygote [or Crossing to BALB/cAJcl]
References
Critical role of plasmacytoid dendritic cells in induction of oral tolerance.
Uto T, Takagi H, Fukaya T, Nasu J, Fukui T, Miyanaga N, Arimura K, Nakamura T, Choijookhuu N, Hishikawa Y, Sato K
J. Allergy Clin. Immunol., (2018). 29477579

Plasmacytoid dendritic cells are crucial for the initiation of inflammation and T cell immunity in vivo.
Takagi H, Fukaya T, Eizumi K, Sato Y, Sato K, Shibazaki A, Otsuka H, Hijikata A, Watanabe T, Ohara O, Kaisho T, Malissen B, Sato K
Immunity, 35, 958-971 (2011). 22177923

Plasmacytoid dendritic cells orchestrate TLR7-mediated innate and adaptive immunity for the initiation of autoimmune inflammation.
Takagi H, Arimura K, Uto T, Fukaya T, Nakamura T, Choijookhuu N, Hishikawa Y, Sato K
Sci. Rep., 6, 24477 (2016). 27075414

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)7EGFP
HBEGFheparin-binding EGF-like growth factor (human)7HBEGF
IRES2internal ribosomal entry site (EMCV)7IRES2
Siglechsialic acid binding Ig-like lectin H7Siglechtargeted mutation 1.1, Katsuaki Sato
loxPphage P1 loxP7loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal circulating cytokine level(MP:0010210)

  • abnormal dendritic cell antigen presentation(MP:0002455)

  • abnormal plasmacytoid dendritic cell physiology(MP:0008769)

  • abnormal regulatory T cell morphology(MP:0004941)

  • decreased T-helper 17 cell number(MP:0010220)
    • more 8 phenotypes
  • decreased circulating interferon-alpha level(MP:0008574)

  • decreased circulating interleukin-12b level(MP:0008648)

  • decreased plasmacytoid dendritic cell number(MP:0008124)

  • decreased susceptibility to bacterial infection induced morbidity/mortality(MP:0009789)

  • decreased susceptibility to infection induced morbidity/mortality(MP:0009786)

  • increased T-helper 17 cell number(MP:0010219)

  • increased circulating interferon-alpha level(MP:0008573)

  • increased circulating interleukin-12b level(MP:0008647)
    • Detailed phenotype data

    Ordering Information

    Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site2 (ires2), human HB-EGF cDNA, Aequorea Victoria EGFP cDNA, phage P1 loxP site, mouse Siglech genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Immunity, 35, 958-971 (2011). Sci. Rep., 6, 24477 (2016).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR (Dr. Katsuaki Sato). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a “MTA for Siglech-IRES2DTREGFP Knock-In Micefor Academic Research Purposes” specified by the DEPOSITOR and Inserm.
    DepositorKatsuaki Sato (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. MTA for Siglech-IRES2DTREGFP Knock-In Mice for Academic Research Purposes (English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Depositor's present address; Division of Immunology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki.
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data