Strain Data Sheet
RBRC09685
Strain Information | |
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| Image | |
| BRC No. | RBRC09685 |
| Type | Chemically-induced Mutation Congenic |
| Species | Mus musculus |
| Strain name | C3B6-Mad2l2<repro22> |
| Former Common name | repro22 |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | C3H/HeJJcl |
| Appearance | |
| Strain development | Developed by the NIH-supported Reproductive Genomics program at JAX. Deposited by Tetsuo Kunieda, Okayama University in 2016. C57BL/6 and C3H mixed genetic backgrounds. |
| Strain description | An ENU-induced mutant line harboring a missense mutation in the 3rd exon of the mouse Mad2l2 (MAD2 mitotic arrest deficient-like 2) gene. Amino acid substitution of arginine for cysteine at position 70 (C70R). Both male and female homozygotes exhibit infertility. Female homozygotes develop ovarian tumors at a high frequency. |
| Colony maintenance | |
| References | Leydig cell hyperplasia in an ENU-induced mutant mouse with germ cell depletion. Khalaj M, Abbasi A R, Nishimura R, Akiyama K, Tsuji T, Noguchi J, Okuda K, Kunieda T J. Reprod. Dev. 54, 225-228, (2008). 18385543A missense mutation in Rev7 disrupts formation of Polzeta, impairing mouse development and repair of genotoxic agent-induced DNA lesions. Khalaj M, Abbasi A, Yamanishi H, Akiyama K, Wakitani S, Kikuchi S, Hirose M, Yuzuriha M, Magari M, Degheidy H A, Abe K, Ogura A, Hashimoto H, Kunieda T J. Biol. Chem., 289, 3811-3824, (2014). 24356953Lack of Rev7 function results in development of tubulostromal adenomas in mouse ovary. Abbasi A, Khalaj M, Akiyama K, Mukai Y, Matsumoto H, Acosta T J, Said N, Yoshida M, Kunieda T Mol. Cell Endocrinol., 412, 19-25, (2015). 26004212 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Mad2l2 MGI:1919140 | MAD2 mitotic arrest deficient-like 2 | 4 | Mad2l2<repro22> MGI:3512941 | reproductive mutant 22, JAX Reproductive Mutagenesis Program | |||
Phenotype | |
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| Phenotype annotation from literatures by Mammalian phenotype ontology | more 14 phenotypes |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | |
| Research application | |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. |
| Depositor | Tetsuo Kunieda (Okayama University) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |