RBRC09682

Information1

Image
BRC No.RBRC09682
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-A930003A15Rik<tm1Tfur>
Former Common nameD15 KO mouse
H-2 Haplotype
ES Cell lineJM8A3 [C57BL/6N-A<tm1Brd>]
Background strain
Appearance
Strain developmentDeveloped by Takahisa Furukawa, Institute for Protein Research, Osaka University in 2015. JM8A3 (C57BL/6N) ES cell line was used. Mice were maintained in the C57BL/6 background.
Strain descriptionA930003A15Rik knockout mice. A930003A15Rik is a long noncoding RNA and highly expressed at the inner retina.
Colony maintenance
References

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
A930003A15RikRIKEN cDNA A930003A15 gene16A930003A15Rik<tm1Tfur>targeted mutation 1, Takahisa Furukawa

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site16Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site16Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP16loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)16mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 PolyA signal16

Information2

Donor DNAyeast FRT (flippase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neomycin resistance gene, SV40 PolyA signal, P1 phage loxP site, mouse A930003A15Rik genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must perform the study on a collaboration basis with the DEPOSITOR.
DepositorTakahisa Furukawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data