RBRC09681

Information1

Image
BRC No.RBRC09681
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Foxf1<tm1.1Tsuka>
Former Common nameFoxf1a conditional knockout mice
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Shin-ichi Tominaga and Tsukasa Ohmori, Jichi Medical University. ES cell line derived from 129S6/SvEvTac background was used. Mice were backcrossed to C57BL/6 for 5 generations.
Strain descriptionFloxed Foxf1a (forkhead box F1) mice. Exon 1 was flanked by a pair of loxP and PGK-Neo was removed by crossing to CAG-Flpe mice.
Colony maintenance
References

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Foxf1forkhead box F18Foxf1<tm1.1Tsuka>targeted mutation 1.1, Tsukasa Ohmori

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site8Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP8loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP8loxP

Information2

Donor DNAyeast Frt (flippase recombination target) site, Phage P1 loxP sites, mouse Foxf1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain an approval from Dr. Tsukasa Ohmori, Jichi Medical University using the Approval Form. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorShin-ichi Tominaga (Jichi Medical University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data