Strain Data Sheet

RBRC09681

Strain Information

Image
BRC No.RBRC09681
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Foxf1<tm1.1Tsuka>
Former Common nameFoxf1a conditional knockout mice
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Shin-ichi Tominaga and Tsukasa Ohmori, Jichi Medical University. ES cell line derived from 129S6/SvEvTac background was used. Mice were backcrossed to C57BL/6 for 5 generations.
Strain descriptionFloxed Foxf1a (forkhead box F1) mice. Exon 1 was flanked by a pair of loxP and PGK-Neo was removed by crossing to CAG-Flpe mice.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Foxf1forkhead box F18Foxf1targeted mutation 1.1, Tsukasa Ohmori
  • alveolar capillary dysplasia with misalignment of pulmonary veins(MedGEN)
    • Frtyeast FRT (flippase recombination target) site8Frt
  • alveolar capillary dysplasia with misalignment of pulmonary veins(MedGEN)
    • loxPphage P1 loxP8loxP
  • alveolar capillary dysplasia with misalignment of pulmonary veins(MedGEN)
    • loxPphage P1 loxP8loxP
  • alveolar capillary dysplasia with misalignment of pulmonary veins(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAyeast Frt (flippase recombination target) site, Phage P1 loxP sites, mouse Foxf1 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain an approval from Dr. Tsukasa Ohmori, Jichi Medical University using the Approval Form. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
    DepositorShin-ichi Tominaga (Jichi Medical University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data