Strain Data Sheet

RBRC09668

Strain Information

Image
BRC No.RBRC09668
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Ndrg2<tm1.1Hori>
Former Common nameNdrg2 Knockout mice
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Osamu Hori, Kanazawa University. ES cell line in 129Sv/J background was used. Mice were backcrossed to C57BL/6 for 8 generations.
Strain descriptionNdrg2 knockout mice. Exon 3 and 4 of the Ndrg2 gene were replaced with a pgk-neo cassette and then it was removed by crossing to CAG-Cre mice.
Colony maintenance
References
Deletion of N-myc downstream-regulated gene 2 attenuates reactive astrogliosis and inflammatory response in a mouse model of cortical stab injury.
Takarada-Iemata M, Kezuka D, Takeichi T, Ikawa M, Hattori T, Kitao Y, Hori O
J. Neurochem., 130, 374-387 (2014). 24697507

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ndrg2N-myc downstream regulated gene 214Ndrg2targeted mutation 1.1, Osamu Hori
loxPphage P1 loxP14loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal astrocyte physiology(MP:0008916)

  • abnormal glial cell physiology(MP:0003690)

  • abnormal microglial cell chemotaxis(MP:0010761)

  • decreased astrocyte number(MP:0012066)

  • decreased inflammatory response(MP:0001876)
    • more 3 phenotypes
  • decreased interleukin-6 secretion(MP:0008706)

  • decreased neuron apoptosis(MP:0003204)

  • decreased susceptibility to injury(MP:0005166)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP site, mouse Ndrg2 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurochem., 130, 374-387 (2014).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from use of the BIOLOGICAL RESOURCE.
    DepositorOsamu　Hori (Kanazawa University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data