RBRC09665

Information1

Image
BRC No.RBRC09665
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6(CB)-Gt(ROSA)26Sor<tm1.1(CAG-AKR1C3)Watk>
Former Common namehPGFS KI
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeveloped by Kikuko Watanabe, Koshien University in 2015. RENKA ES cell line (C57BL/6N) was used. Mice were maintained in C57BL/6 and CBA mixed background.
Strain descriptionKnock-in mice for overexpression of human AKR1C3. Targeted insertion of CAG-loxP-CAT-FRT-PGK-neo-FRT-loxP-FAM213B-pA cassette into the mouse Rosa26 locus.  A DNA fragment of loxP-CAT-FRT-PGK-neo-FRT-loxP was removed by crossing to B6;CBA-Tg(CAG-Cre)47Imeg.
Colony maintenance
ReferencesGenes Dev., 5, 1513-1523 (1991). 1653172
FEBS Lett., 462, 335-340 (1999). 10622721
Prostaglandins Other Lipid Mediat., 68-69:401-407 (2002). 12432932

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1.1(CAG-AKR1C3)Watk>targeted mutation 1.1, Kikuko Watanabe

Gene symbolGene nameChr.Allele symbolAllele namePromoter
AKR1C3human ortholog mouse Akr1c18 (MGI: 2145420), aldo-keto reductase family 1, member C186AKR1C3CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Information2

Donor DNAマウス(Mus musculus) ROSA26, ヒト(Homo Sapiens) hPGFS,ウシ(Bos taurus) bovine growth hormone polyA signal, サイトメガロウィルス(cytomegalovirus) cytomegalovirus enhancer, ニワトリ(Gallus gallus) β-actin promoter, ウサギ(Oryctolagus cuniculus)β-globin exon, intron, ファージ P1 (Enterobacteria phage P1) Lox-P
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FEBS lett., 462, 335-340 (1999).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the Dr. Kikuko Watanabe is requested.
DepositorKikuko Watanabe (Kansai Medical University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.

BRC mice in Publications

No Data