Strain Data Sheet

RBRC09659

Strain Information

Image
BRC No.RBRC09659
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6JJcl-Fap<em1Fjw>
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Wataru Fujii, Graduate School of Agricultural and Life Sciences, The University Tokyo, and Akira Ishikawa, Graduate School of Bioagricultural Science, Nagoya University in 2015.
Strain descriptionMice generated by the CRISPR/Cas9, which deleted a genomic fragment corresponding to exon 4 and 5 of Fap (fibroblast activation protein). Homozygous mice are viable and fertile.
Colony maintenanceHeterozygote x C57BL/6JJcl.
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Fapfibroblast activation protein2Fapendonuclease-mediated mutation 1, Wataru Fujii

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNA
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of its use with the DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
    DepositorAkira Ishikawa (Nagoya University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data