RBRC09657

Information1

Image
BRC No.RBRC09657
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Clec4a4<tm1.1Ksat>
Former Common nameClec4a4-/- mice
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Katsuaki Sato, RIKEN Research Center for Allergy and Immunology in 2016. Bruce 4 ES cells derived from B6.Cg-Thy1<a> were used. Mice were backcrossed to C57BL/6J for 9 generations.
Strain descriptionClec4A4 (DCIR2) knockout mice. IRES-EGFP cassette was replaced with exon 1 and exon 2 of the Clec4A4 gene. No GFP expression was detected in the mice.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesNat. Commun., 7, 11273(2016). 27068492

Health Report

Examination Date / Room / Rack2017/10/02Room:3-2Rack:D
2017/08/21Room:3-2Rack:D
2017/06/05Room:3-2Rack:D
2017/04/03Room:3-2Rack:D
2017/02/27Room:3-2Rack:D
2017/02/27Room:3-2Rack:D
2017/02/06Room:3-2Rack:D
2016/12/05Room:3-2Rack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Clec4a4C-type lectin domain family 4, member a46Clec4a4<tm1.1Ksat>targeted mutation 1.1, Katsuaki Sato

Gene symbolGene nameChr.Allele symbolAllele namePromoter
EGFPEnhanced Green Fluorescent Protein, F64L and S65T (Aequorea victoria)6EGFP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Ires2internal ribosomal entry site (EMCV)6Ires2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Information2

Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site 2 (ires2), Aequorea Victoria EGFP cDNA, phage P1 loxP site, mouse Clec4a4 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Commun., 7, 11273 (2016).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR (Dr. Katsuaki Sato). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a “MTA for Clec4a4-IRES2EGFP KI transgenic mice for Academic Research Purposes” specified by the DEPOSITOR and Inserm.
DepositorKatsuaki Sato (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
MTA for Clec4a4-IRES2EGFP Knock-In Mice for Academic Research Purposes (English)
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Depositor's present address; Division of Immunology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki.

BRC mice in Publications

No Data