Strain Data Sheet

RBRC09657

Strain Information

Image
BRC No.RBRC09657
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Clec4a4<tm1.1Ksat>
Former Common nameClec4a4-/- mice
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Katsuaki Sato, RIKEN Research Center for Allergy and Immunology in 2016. Bruce 4 ES cells derived from B6.Cg-Thy1<a> were used. Mice were backcrossed to C57BL/6J for 9 generations.
Strain descriptionClec4A4 (DCIR2) knockout mice. IRES-EGFP cassette was replaced with exon 1 and exon 2 of the Clec4A4 gene. No GFP expression was detected in the mice.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
Clec4A4 is a regulatory receptor for dendritic cells that impairs inflammation and T-cell immunity.
Uto T, Fukaya T, Takagi H, Arimura K, Nakamura T, Kojima N, Malissen B, Sato K
Nat. Commun., 7, 11273（2016）. 27068492

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Clec4a4C-type lectin domain family 4, member a46Clec4a4targeted mutation 1.1, Katsuaki Sato
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)6EGFP
IRES2internal ribosomal entry site (EMCV)6IRES2
loxPphage P1 loxP6loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site 2 (ires2), Aequorea Victoria EGFP cDNA, phage P1 loxP site, mouse Clec4a4 genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Commun., 7, 11273 (2016).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR (Dr. Katsuaki Sato). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a “MTA for Clec4a4-IRES2EGFP KI transgenic mice for Academic Research Purposes” specified by the DEPOSITOR and Inserm.
    DepositorKatsuaki Sato (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. MTA for Clec4a4-IRES2EGFP Knock-In Mice for Academic Research Purposes (English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Depositor's present address; Division of Immunology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki.
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data