Strain Data Sheet

RBRC09650

Strain Information

Image
BRC No.RBRC09650
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Slc1a2-G-CaMP7)Bsi
Former Common nameGLT1-G-CaMP7, G7NG817
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Hajime Hirase, Brain Science Institute, RIKEN in 2016. A BAC DNA construct was injected to fertilized eggs derived from C57BL/6J. Mice were maintained in this genetic background. Homozygous mice are viable and fertile.
Strain descriptionG-CaMP7 transgenic mice under the control of Slc1a2 (GLT-1) promoter. Astrocytes and a subset of cortical neurons express G-CaMP7.
Colony maintenance
References
Adrenergic receptor antagonism induces neuroprotection and facilitates recovery from acute ischemic stroke.
Monai H, Wang X, Yahagi K, Lou N, Mestre H, Xu Q, Abe Y, Yasui M, Iwai Y, Nedergaard M, Hirase H
Proc. Natl. Acad. Sci. USA, 116(22):11010-11019 (2019). 31097598

Adrenergic inhibition facilitates normalization of extracellular potassium after cortical spreading depolarization.
Monai H, Koketsu S, Shinohara Y, Ueki T, Kusk P, Hauglund N L, Samson A J, Nedergaard M, Hirase H
Sci. Rep., 11, 8150 (2021). 33854148

Variations in promoter activity reveal a differential expression and physiology of glutamate transporters by glia in the developing and mature CNS.
Regan M R, Huang Y H, Kim Y S, Dykes-Hoberg M I, Jin L, Watkins A M, Bergles D E, Rothstein J D
J. Neurosci., 20;27, 6607-6619 (2007). 17581948

Calcium imaging reveals glial involvement in transcranial direct current stimulation-induced plasticity in mouse brain.
Monai H, Ohkura M, Tanaka M, Oe Y, Konno A, Hirai H, Mikoshiba K, Itohara S, Nakai J, Iwai Y, Hirase H
Nat Commun. (2016) 7:11100. DOI: 10.1038/ncomms11100 27000523

Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals.
Ohkura M, Sasaki T, Sadakari J, Gengyo-Ando K, Kagawa-Nagamura Y, Kobayashi C, Ikegaya Y, Nakai J
PLoS One, 7, e51286, (2012). 23240011

Health Report

Examination Date / Room / Rack2024/04/08Room:3-BRack:F
2024/01/09Room:3-BRack:F
2023/10/10Room:3-BRack:F
2023/07/10Room:3-BRack:F
2023/04/10Room:3-BRack:F

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frtyeast FRT (flippase recombination target) siteUNFrt
  • developmental and epileptic encephalopathy, 41(MedGEN)
    • GCaMP7RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNAUNGCaMP7mouse Slc1a2 promoter
  • developmental and epileptic encephalopathy, 41(MedGEN)
    • HBB2rabbit beta-globin polyAUNHBB2
  • developmental and epileptic encephalopathy, 41(MedGEN)
    • WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)UNWPRE
  • developmental and epileptic encephalopathy, 41(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Slc1a2 genomic DNA, GCaMP7 (RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA), woodchuck hepatitis virus WPRE (woodchuck hepatitis virus posttranscriptional regulatory element) sequence, rabbit Beta globin polyA, yeast FRT (flippase recombination target) site, Phage P1 loxP site derived from pBACe3.6 vector, BAC clone RP23-361H22
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the above literature designated by the DEPOSITOR is recommended. Nat Commun. (2016) 7:11100. DOI: 10.1038/ncomms11100
    DepositorHajime Hirase (RIKEN)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (3 to 6 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Aug 2017

    BRC mice in Publications

    Holstein-Rønsbo S, Gan Y, Giannetto MJ, Rasmussen MK, Sigurdsson B, Beinlich FRM, Rose L, Untiet V, Hablitz LM, Kelley DH, Nedergaard M.
    Glymphatic influx and clearance are accelerated by neurovascular coupling.
    Nat Neurosci (2023) 37264158
    Monai H, Wang X, Yahagi K, Lou N, Mestre H, Xu Q, Abe Y, Yasui M, Iwai Y, Nedergaard M, Hirase H.
    Adrenergic receptor antagonism induces neuroprotection and facilitates recovery from acute ischemic stroke.
    Proc Natl Acad Sci U S A 116(22) 11010-11019(2019) 31097598