Strain Data Sheet
RBRC09650
Information1 | |
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| Image | |
| BRC No. | RBRC09650 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | C57BL/6-Tg(Slc1a2-G-CaMP7)Bsi |
| Former Common name | GLT1-G-CaMP7, G7NG817 |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | C57BL/6JJcl |
| Appearance | |
| Strain development | Developed by Hajime Hirase, Brain Science Institute, RIKEN in 2016. A BAC DNA construct was injected to fertilized eggs derived from C57BL/6J. Mice were maintained in this genetic background. Homozygous mice are viable and fertile. |
| Strain description | G-CaMP7 transgenic mice under the control of Slc1a2 (GLT-1) promoter. Astrocytes and a subset of cortical neurons express G-CaMP7. |
| Colony maintenance | |
| References | Proc. Natl. Acad. Sci. USA, 116(22):11010-11019 (2019). 31097598 J. Neurosci., 20;27, 6607-6619 (2007). 17581948 Nat Commun. (2016) 7:11100. DOI: 10.1038/ncomms11100 27000523 Sci. Rep., 11, 8150 (2021). 33854148 PLoS One, 7, e51286, (2012). 23240011 |
Health Report | |
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| Examination Date / Room / Rack | 2023/07/10Room:3-BRack:F 2023/04/10Room:3-BRack:F |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) siteUNFrt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GCaMP7RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNAUNGCaMP7mouse Slc1a2 promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HBB2rabbit beta-globin polyAUNHBB2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)UNWPRE |
Information2 | |
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| Donor DNA | mouse Slc1a2 genomic DNA, GCaMP7 (RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA), woodchuck hepatitis virus WPRE (woodchuck hepatitis virus posttranscriptional regulatory element) sequence, rabbit Beta globin polyA, yeast FRT (flippase recombination target) site, Phage P1 loxP site derived from pBACe3.6 vector, BAC clone RP23-361H22 |
| Research application | Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the above literature designated by the DEPOSITOR is recommended. Nat Commun. (2016) 7:11100. DOI: 10.1038/ncomms11100 |
| Depositor | Hajime Hirase (RIKEN) |
| Strain Status |  Live mice Frozen embryos Frozen sperm |
| Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (3 to 6 months) |
| Additional Info. | Mouse of the Month Aug 2017 Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Monai H, Wang X, Yahagi K, Lou N, Mestre H, Xu Q, Abe Y, Yasui M, Iwai Y, Nedergaard M, Hirase H. Adrenergic receptor antagonism induces neuroprotection and facilitates recovery from acute ischemic stroke. Proc Natl Acad Sci U S A 116(22) 11010-11019(2019) 31097598 |