RBRC09650

Information1

Image
BRC No.RBRC09650
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Slc1a2-G-CaMP7)Bsi
Former Common nameGLT1-G-CaMP7, G7NG817
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Hajime Hirase, Brain Science Institute, RIKEN in 2016. A BAC DNA construct was injected to fertilized eggs derived from C57BL/6J. Mice were maintained in this genetic background. Homozygous mice are viable and fertile.
Strain descriptionG-CaMP7 transgenic mice under the control of Slc1a2 (GLT-1) promoter. Astrocytes and a subset of cortical neurons express G-CaMP7.
Colony maintenance
ReferencesPLoS One 7,e51286 (2012). 23240011
J. Neurosci., 20;27, 6607-6619 (2007). 17581948
Proc. Natl. Acad. Sci. USA, 116(22):11010-11019 (2019). 31097598
Nat Commun. (2016) 7:11100. DOI: 10.1038/ncomms11100 27000523

Health Report

Examination Date / Room / Rack2018/12/17Room:3-BRack:L
2018/10/15Room:3-BRack:L
2018/08/20Room:3-BRack:L
2018/06/18Room:3-BRack:L
2018/04/16Room:3-BRack:L
2018/03/12Room:3-BRack:L
2018/03/12Room:3-BRack:L
2018/02/19Room:3-BRack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) siteUNFrt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GCaMP7RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNAUNGCaMP7mouse Slc1a2 promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
HBB2rabbit beta-globin polyAUNHBB2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)UNWPRE

Information2

Donor DNAmouse Slc1a2 genomic DNA, GCaMP7 (RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA), woodchuck hepatitis virus WPRE (woodchuck hepatitis virus posttranscriptional regulatory element) sequence, rabbit Beta globin polyA, yeast FRT (flippase recombination target) site, Phage P1 loxP site derived from pBACe3.6 vector, BAC clone RP23-361H22
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the above literature designated by the DEPOSITOR is recommended. Nat Commun. (2016) 7:11100. DOI: 10.1038/ncomms11100
DepositorHajime Hirase (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Mouse of the Month Aug 2017

BRC mice in Publications

No Data