RBRC09649

Information1

Image
BRC No.RBRC09649
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Htr5a<tm2Ahky>
Former Common nameHtr5A-flox
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Akihiro Yamanaka, Nagoya University. ES cells derived from C57BL/6N were used. Mice were maintained in C57BL/6 background.
Strain descriptionFloxed Htr5a mice. In the Htr5a locus, one loxP site was placed upstream of Exon2 and the other was after the Stop codon. A pgk-neo cassette still remains next to the 5’loxP site.
Colony maintenance
References

Health Report

Examination Date / Room / Rack2020/04/06Room:3-5Rack:B
2020/02/03Room:3-5Rack:B
2019/12/02Room:3-5Rack:B
2019/10/16Room:3-5Rack:B
2019/08/05Room:3-5Rack:B
2019/06/03Room:3-5Rack:B
2019/04/01Room:3-5Rack:B
2019/02/04Room:3-5Rack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Htr5a5-hydroxytryptamine (serotonin) receptor 5A5Htr5a<tm2 Ahky>targeted mutation 2, Akihiro Yamanaka

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site5Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site5Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)5mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAphage P1 loxP sites, yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse pgk-PolyA, mouse Htr5a genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. 後日指定。(will be announced).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorAkihiro Yamanaka (Nagoya University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityLive mouse (1 to 3 months)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data