Strain Data Sheet

RBRC09647

Strain Information

Image
BRC No.RBRC09647
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;Cg-Notch2<tm1.1Hhi>
Former Common nameNotch2 flox
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Shigeru Chiba, University of Tsukuba. TT2 ES [(C57BL/6NCrlj x CBA/JNCrlj)F1] was used. C57BL/6N and CBA mixed background.
Strain descriptionFolxed Notch2 mice. A pair of loxP sites are flanking exons encoding transmembrane domain of the Notch2 gene.
Colony maintenance
References
Notch2 is preferentially expressed in mature B cells and indispensable for marginal zone B lineage development.
Saito T, Chiba S, Ichikawa M, Kunisato A, Asai T, Shimizu K, Yamaguchi T, Yamamoto G, Seo S, Kumano K, Nakagami-Yamaguchi E, Hamada Y, Aizawa S, Hirai H
Immunity, 18, 675-685 (2003). 12753744

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Notch2notch 23Notch2targeted mutation 1.1, Hisamaru Hirai
  • Acroosteolysis dominant type(DisGeNET, MedGEN)

  • Alagille syndrome due to a NOTCH2 point mutation(MedGEN)
    • loxPphage P1 loxP3loxP
  • Acroosteolysis dominant type(DisGeNET, MedGEN)

  • Alagille syndrome due to a NOTCH2 point mutation(MedGEN)
    • loxPphage P1 loxP3loxP
  • Acroosteolysis dominant type(DisGeNET, MedGEN)

  • Alagille syndrome due to a NOTCH2 point mutation(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, mouse Notch2 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Immunity, 18, 675-685 (2003). For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
    DepositorShigeru Chiba (University of Tsukuba)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data