Strain Data Sheet

RBRC09646

Strain Information

Image
BRC No.RBRC09646
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Rftn2<tm1b(EUCOMM)Mmtm>
Former Common nameRaftlin2 KO
H-2 Haplotype
ES Cell lineEGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Misako Matsumoto, Hokkaido University in 2013. A FRT-flanked neo cassette was removed by a CAG-Cre line. C57BL/6 background.
Strain descriptionRftn2 KO mice. Exon 2 of the Rftn2 gene was deleted.
Colony maintenance
References
Raftlin Controls Lipopolysaccharide-Induced TLR4 Internalization and TICAM-1 Signaling in a Cell Type-Specific Manner.
Tatematsu M, Yoshida R, Morioka Y, Ishii N, Funami K, Watanabe A, Saeki K, Seya T, Matsumoto M
J. Immunol., 196(9):3865-76 (2016) 27022195

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)1En2
Frtyeast FRT (flippase recombination target) site1Frt
Iresinternal ribosomal entry site (EMCV)1
Rftn2raftlin family member 21Rftn2targeted mutation 1b, Misako Matsumoto
lacZbeta-galactosidase (E. coli)1
loxPphage P1 loxP1loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • decreased interferon-beta secretion(MP:0008565)
    • Detailed phenotype data

    Ordering Information

    Donor DNAyeast FRT (flipase recombination target) site, Mouse En2 intron 2/exon 3 splice acceptor sequence, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, SV40 polyadenylation site, Bacteriophage P1 loxP site, mouse Rftn2 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Immunol., 196(9):3865-76 (2016).The RECIPIENT shall acknowledge the EUCOMM as the provider of the knockout vector.
    DepositorMisako Matsumoto (Hokkaido University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data