RBRC09646

Information1

Image
BRC No.RBRC09646
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Rftn2<tm1b(EUCOMM)Mmtm>
Former Common nameRaftlin2 KO
H-2 Haplotype
ES Cell lineEGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Misako Matsumoto, Hokkaido University in 2013. A FRT-flanked neo cassette was removed by a CAG-Cre line. C57BL/6 background.
Strain descriptionRftn2 KO mice. Exon 2 of the Rftn2 gene was deleted.
Colony maintenance
ReferencesJ. Immunol., 196(9):3865-76 (2016) 27022195

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Rftn2raftlin family member 21Rftn2<tm1bMmtm>targeted mutation 1b, Misako Matsumoto

Gene symbolGene nameChr.Allele symbolAllele namePromoter
En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)1En2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site1Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Iresinternal ribosomal entry site (EMCV)1

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)1

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP1loxP

Information2

Donor DNAyeast FRT (flipase recombination target) site, Mouse En2 intron 2/exon 3 splice acceptor sequence, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, SV40 polyadenylation site, Bacteriophage P1 loxP site, mouse Rftn2 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Immunol., 196(9):3865-76 (2016).The RECIPIENT shall acknowledge the EUCOMM as the provider of the knockout vector.
DepositorMisako Matsumoto (Hokkaido University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.

BRC mice in Publications

No Data