Information1 | |
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Image | |
BRC No. | RBRC09645 |
Type | Transgene![]() |
Species | Mus musculus |
Strain name | B6-Tg(Slc32a1-YFP*)39Yyan |
Former Common name | |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Yuchio Yanagawa, Gunma University Graduate School of Medicine. A BAC DNA construct was injected to fertilized eggs derived from C57BL/6. Mice were maintained in this genetic background. |
Strain description | BAC transgenic mice expressing Venus under the control of Slc32a1 (VGAT) promoter. Venus expression is sufficiently bright to visualize inhibitory neurons. |
Colony maintenance | Carrier x Noncarrier [or C57BL/6JJcl] |
References | Neuroscience., 164, 1031-1043 (2009). 19766173 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Slc32a1solute carrier family 32 (GABA vesicular transporter), member 1UNSlc32a1 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter VenusYellow Fluorescent Protein (Aequorea victoria)UNVenusmouse Vesicular GABA transporter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter catE. coli chloramphenicol acetyltransferase (CAT)UN Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPUNloxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyAUN |
Information2 | |
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Donor DNA | mouse Vesicular GABA transporter (Ebihara et al., 2003), jellyfish Venus (improved version of YFP) cDNA (Nagai et al., 2002), SV40 virus SV40 polyA, E. coli Chloramphenicol acetyltransferase (CAT), E. coli lacZ alpha, Phage P1 loxP derived from pBeloBAC11 vector |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature is requested. Neuroscience 164, 1031-1043, 2009. The BIOLOGICAL RESOURCE should not be redistributed without a permission of the DEPOSITOR scientist. The use of the BIOLOGICAL RESOURCE is only for academic researches but not for profit organizations. The Venus gene (hereinafter the MATERIAL) incorporated into the BIOLOGICAL RESOURCE was supplied by Dr. Atsushi Miyawaki, Laboratory for Cell Function Dynamics, RIKEN Brain Science Institute, 2-1 Hirosawa, Wako, Saitama, 351-0198, JAPAN (email: matsushi@brain.riken.jp). The RECIPIENT agrees to acknowledge Dr. Miyawaki (Sample: “Venus cDNA was developed by Dr. Atsushi Miyawaki at RIKEN, Wako, Japan.”) and submit Dr. Miyawaki the intended publications in oral or written forms as related to the MATERIAL prior to submission and send Dr. Miyawaki the reprints. |
Depositor | Yuchio Yanagawa (Gunma University) |
Strain Status | ![]() ![]() |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |