Strain Information | |
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Image | |
BRC No. | RBRC09645 |
Type | Transgene![]() |
Species | Mus musculus |
Strain name | B6-Tg(Slc32a1-YFP*)39Yyan |
Former Common name | |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Yuchio Yanagawa, Gunma University Graduate School of Medicine. A BAC DNA construct was injected to fertilized eggs derived from C57BL/6. Mice were maintained in this genetic background. |
Strain description | BAC transgenic mice expressing Venus under the control of Slc32a1 (VGAT) promoter. Venus expression is sufficiently bright to visualize inhibitory neurons. |
Colony maintenance | Carrier x Noncarrier [or C57BL/6JJcl] |
References | Fluorescent labeling of both GABAergic and glycinergic neurons in vesicular GABA transporter (VGAT)-venus transgenic mouse. Wang Y, Kakizaki T, Sakagami H, Saito K, Ebihara S, Kato M, Hirabayashi M, Saito Y, Furuya N, Yanagawa Y Neuroscience., 164, 1031-1043 (2009). 19766173 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Slc32a1 | solute carrier family 32 (GABA vesicular transporter), member 1 | UN | Slc32a1 | ||||
Venus | Yellow Fluorescent Protein (Aequorea victoria) | UN | Venus | mouse Vesicular GABA transporter | |||
cat | E. coli chloramphenicol acetyltransferase (CAT) | UN | |||||
loxP | phage P1 loxP | UN | loxP | ||||
SV40 polyA | UN |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | mouse Vesicular GABA transporter (Ebihara et al., 2003), jellyfish Venus (improved version of YFP) cDNA (Nagai et al., 2002), SV40 virus SV40 polyA, E. coli Chloramphenicol acetyltransferase (CAT), E. coli lacZ alpha, Phage P1 loxP derived from pBeloBAC11 vector |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuroscience., 164, 1031-1043 (2009). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The BIOLOGICAL RESOURCE should not be redistributed without a permission of the DEPOSITOR scientist. The use of the BIOLOGICAL RESOURCE is only for academic researches but not for profit organizations. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. Nat. Biotechnol., 20, 87-90 (2002). |
Depositor | Yuchio Yanagawa (Gunma University) |
Strain Status | ![]() ![]() |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |