Strain Data Sheet

RBRC09645

Strain Information

Image
BRC No.RBRC09645
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6-Tg(Slc32a1-YFP*)39Yyan
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Yuchio Yanagawa, Gunma University Graduate School of Medicine. A BAC DNA construct was injected to fertilized eggs derived from C57BL/6. Mice were maintained in this genetic background.
Strain descriptionBAC transgenic mice expressing Venus under the control of Slc32a1 (VGAT) promoter. Venus expression is sufficiently bright to visualize inhibitory neurons.
Colony maintenanceCarrier x Noncarrier [or C57BL/6JJcl]
References
Fluorescent labeling of both GABAergic and glycinergic neurons in vesicular GABA transporter (VGAT)-venus transgenic mouse.
Wang Y, Kakizaki T, Sakagami H, Saito K, Ebihara S, Kato M, Hirabayashi M, Saito Y, Furuya N, Yanagawa Y
Neuroscience., 164, 1031-1043 (2009). 19766173

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Slc32a1 solute carrier family 32 (GABA vesicular transporter), member 1UNSlc32a1
Venus Yellow Fluorescent Protein (Aequorea victoria)UNVenus mouse Vesicular GABA transporter
cat E. coli chloramphenicol acetyltransferase (CAT)UN
loxP phage P1 loxPUNloxP
SV40 polyAUN

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Vesicular GABA transporter (Ebihara et al., 2003), jellyfish Venus (improved version of YFP) cDNA (Nagai et al., 2002), SV40 virus SV40 polyA, E. coli Chloramphenicol acetyltransferase (CAT), E. coli lacZ alpha, Phage P1 loxP derived from pBeloBAC11 vector
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuroscience., 164, 1031-1043 (2009). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The BIOLOGICAL RESOURCE should not be redistributed without a permission of the DEPOSITOR scientist. The use of the BIOLOGICAL RESOURCE is only for academic researches but not for profit organizations. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
    Nat. Biotechnol., 20, 87-90 (2002).
    DepositorYuchio Yanagawa (Gunma University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data