Strain Data Sheet

RBRC09645

Information1

Image
BRC No.RBRC09645
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6-Tg(Slc32a1-YFP*)39Yyan
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Yuchio Yanagawa, Gunma University Graduate School of Medicine. A BAC DNA construct was injected to fertilized eggs derived from C57BL/6. Mice were maintained in this genetic background.
Strain descriptionBAC transgenic mice expressing Venus under the control of Slc32a1 (VGAT) promoter. Venus expression is sufficiently bright to visualize inhibitory neurons.
Colony maintenanceCarrier x Noncarrier [or C57BL/6JJcl]
ReferencesNeuroscience., 164, 1031-1043 （2009）. 19766173

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Slc32a1solute carrier family 32 (GABA vesicular transporter), member 1UNSlc32a1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
VenusYellow Fluorescent Protein (Aequorea victoria)UNVenusmouse Vesicular GABA transporter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
catE. coli chloramphenicol acetyltransferase (CAT)UN

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyAUN

Information2

Donor DNAmouse Vesicular GABA transporter (Ebihara et al., 2003), jellyfish Venus (improved version of YFP) cDNA (Nagai et al., 2002), SV40 virus SV40 polyA, E. coli Chloramphenicol acetyltransferase (CAT), E. coli lacZ alpha, Phage P1 loxP derived from pBeloBAC11 vector
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature is requested. Neuroscience 164, 1031-1043, 2009. The BIOLOGICAL RESOURCE should not be redistributed without a permission of the DEPOSITOR scientist. The use of the BIOLOGICAL RESOURCE is only for academic researches but not for profit organizations. The Venus gene (hereinafter the MATERIAL) incorporated into the BIOLOGICAL RESOURCE was supplied by Dr. Atsushi Miyawaki, Laboratory for Cell Function Dynamics, RIKEN Brain Science Institute, 2-1 Hirosawa, Wako, Saitama, 351-0198, JAPAN (email: matsushi@brain.riken.jp). The RECIPIENT agrees to acknowledge Dr. Miyawaki (Sample: “Venus cDNA was developed by Dr. Atsushi Miyawaki at RIKEN, Wako, Japan.”) and submit Dr. Miyawaki the intended publications in oral or written forms as related to the MATERIAL prior to submission and send Dr. Miyawaki the reprints.
DepositorYuchio Yanagawa (Gunma University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data