Strain Data Sheet

RBRC09630

Strain Information

Image
BRC No.RBRC09630
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Haus6<tm1.1>
Former Common nameHAUS6 flox
H-2 Haplotype
ES Cell lineHK3i [C57BL/6N]
Background strain
Appearance
Strain developmentDeveloped by Sadanori Watanabe, Gohta Goshima (Nagoya University), Go Shioi, Yasuhide Furuta (RIKEN CLST LARGE). The PGK-neo cassette was deleted by crossing with a mouse expressing FLPe(B6-Tg(CAG-FLPe)36Ito/ItoRbrc (RBRC01834)). HK3i ES cell (C57BL/6N background) was used to generate Haus6 conditional KO mice (Haus6 flox Neo mice)(Accession No. CDB1218K). Haus6 conditional KO mice (Haus6 flox Neo mice)(Accession No. CDB1218K) were crossed with C57BL/6-Tg(CAG-flpe)36Ito/ItoRbrc (RBRC01834), and the resultant mice without the PGK-neo cassette (Haus6 flox mice) were maintained by heterozygous crossing(C57BL/6N background).
Strain descriptionHaus6 (Aug6/Dgt6/Fam29A) gene floxed mice. Haus6 (Aug6/Dgt6/Fam29A) is one of augmin subunits, which mediates microtubule-dependent microtubule nucleation (i.e. amplification). Exon 1 of Haus6 was flanked by loxP sites (Ref: Watanabe S et al Cell Rep 2016). The Neo cassette flanked by FRT sites was deleted by crossing with mice expressing a FLP recombinase (CAG-FLPe). Tissue-specific deletion of HAUS6 can be generated by crossing with various Cre-expressing mice. Homozygous Haus6 KO mice are lethal in early development (Ref: Watanabe S et al Cell Rep 2016). Homozygous Haus6 flox mice are viable.
Colony maintenance
References
Intra-spindle Microtubule Assembly Regulates Clustering of Microtubule-Organizing Centers during Early Mouse Development.
Watanabe S, Shioi G, Furuta Y, Goshima G
Cell Rep., 15(1):54-60 (2016). 27052165

High-efficiency CAG-FLPe deleter mice in C57BL/6J background.
Kanki H, Suzuki H, Itohara S
Exp. Anim., 55, 137-141 (2006). 16651697

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frtyeast FRT (flippase recombination target) site4Frt
Haus6HAUS augmin-like complex, subunit 64Haus6targeted mutation 1.1
loxPphage P1 loxP4loxP
loxPphage P1 loxP4loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal embryo development(MP:0001672)

  • abnormal mitotic spindle morphology(MP:0009760)

  • embryonic lethality, complete penetrance(MP:0011092)
    • Detailed phenotype data

    Ordering Information

    Donor DNAP1 Phage loxP, Yeast frt
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Rep., 15(1):54-60 (2016). Exp. Anim., 55, 137-141 (2006).In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state Accession No.CDB1354K and URL https://large.riken.jp/distribution/mutant-list.html of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, descriptions of the following mice as the source of the BIOLOGICAL RESOURCE are requested in the Materials and Methods section.(Haus6 mice: Acc. No. CDB1218K, https://large.riken.jp/distribution/mutant-list.html and B6-Tg(CAG-FLPe)36 mouse(RBRC01834)(B6-Tg(CAG-FLPe)36 was provided by the RIKEN BRC through the National Bio-Resource Project of the MEXT, Japan). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must contact the DEPOSITOR prior to submission of the publication and include Sadanori Watanabe,the developer of the BIOLOGICAL RESOURCE,as a co-author when the publication is the first report using the BIOLOGICAL RESOURCE. The RECIPIENT must submit any and all copy of the publications using BIOLOGICAL RESOURCES to RIKEN Center for Life Sciences Technologies, Laboratories of Animal Resource development and Genetic Engineering (RIKEN CLST/LARGE) (mutant.bdr@riken.jp). The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. 1. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
    DepositorGohta Goshima (Nagoya University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data