Information1 | |
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Image | |
BRC No. | RBRC09616 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | B6.Cg-Pdgfra<tm1.1(EGFP/cre/ERT2)Hyma> |
Former Common name | PDGFRα-GFPCreERT2 |
H-2 Haplotype | |
ES Cell line | KTPU8 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | |
Strain description | |
Colony maintenance | |
References | Genesis., 53, 329-336 (2015). 25884589 |
Health Report | |
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Examination Date / Room / Rack | 2023/02/20Room:4-ARack:E 2023/02/20Room:4-ARack:E 2022/11/21Room:4-ARack:E 2022/08/22Room:4-ARack:E 2022/05/23Room:4-ARack:E 2022/03/07Room:4-ARack:E 2021/12/06Room:4-ARack:E 2021/10/01Room:4-ARack:E 2021/09/08Room:4-ARack:E 2021/06/07Room:4-ARack:E |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Pdgfraplatelet derived growth factor receptor, alpha polypeptide5Pdgfra<tm1.1GFP/cre/ERT2Hyma>targeted mutation 1.1, Hiroyuki Miwa Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter ERT2estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)5ERT2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site5Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GFPGreen Fluorescent Protein (Aequorea victoria)5 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter crePhage P1 Cre recombinase5cre |
Information2 | |
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Donor DNA | Jellyfish GFP cDNA, Phage P1 Cre recombinase cDNA, human Estrogen receptor binding domain (ERT2)cDNA, yeast FRT (flipase recombination target) site, mouse Pdgfra genomic DNA |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. |
Depositor | Takumi Era (Kumamoto University) |
Strain Status | ![]() ![]() ![]() |
Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
Additional Info. | Mouse of the Month Sep 2016 Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Kimura K, Ramirez K, Nguyen TAV, Yamashiro Y, Sada A, Yanagisawa H. Contribution of PDGFRα-positive cells in maintenance and injury responses in mouse large vessels. Sci Rep 11(1) 8683(2021) 33883668 |