Strain Data Sheet

RBRC09616

Strain Information

Image
BRC No.RBRC09616
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Pdgfra<tm1.1(EGFP/cre/ERT2)Hyma>
Former Common namePDGFRα-GFPCreERT2
H-2 Haplotype
ES Cell lineKTPU8 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6JJcl
Appearance
Strain development
Strain description
Colony maintenance
References
Generation and characterization of PDGFRalpha-GFPCreERT2 knock-In mouse line.
Miwa H, Era T
Genesis., 53, 329-336 (2015). 25884589

Health Report

Examination Date / Room / Rack2025/02/17Room:4-ARack:ESentinel mouse program
2024/11/18Room:4-ARack:ESentinel mouse program
2024/08/19Room:4-ARack:ESentinel mouse program
2024/05/20Room:4-ARack:ESentinel mouse program
2024/02/19Room:4-ARack:ESentinel mouse program
2023/11/20Room:4-ARack:ESentinel mouse program
2023/08/21Room:4-ARack:ESentinel mouse program
2023/05/22Room:4-ARack:ESentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
ERT2 estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)5ERT2
Frt yeast FRT (flippase recombination target) site5Frt
GFP Green Fluorescent Protein (Aequorea victoria)5
Pdgfra
MGI:97530		platelet derived growth factor receptor, alpha polypeptide5Pdgfra<tm1.1GFP/cre/ERT2Hyma>
MGI:5644346		targeted mutation 1.1, Hiroyuki Miwa
  • gastrointestinal stromal tumor(DisGeNET)

  • idiopathic hypereosinophilic syndrome(MedGEN)

  • polyps, multiple and recurrent inflammatory fibroid, gastrointestinal(MedGEN)
    • cre Phage P1 Cre recombinase5cre

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • cellular phenotype(MP:0005384)

  • growth/size/body region phenotype(MP:0005378)
    • Detailed phenotype data

    Ordering Information

    Donor DNAJellyfish GFP cDNA, Phage P1 Cre recombinase cDNA, human Estrogen receptor binding domain (ERT2)cDNA, yeast FRT (flipase recombination target) site, mouse Pdgfra genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Cre driver
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorTakumi Era (Kumamoto University)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Cre-ERT(2): NOTICE TO RECIPIENT
    5. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Sep 2016

    BRC mice in Publications

    Nawaz A, Bilal M, Fujisaka S, Kado T, Aslam MR, Ahmed S, Okabe K, Igarashi Y, Watanabe Y, Kuwano T, Tsuneyama K, Nishimura A, Nishida Y, Yamamoto S, Sasahara M, Imura J, Mori H, Matzuk MM, Kudo F, Manabe I, Uezumi A, Nakagawa T, Oishi Y, Tobe K.
    Depletion of CD206+ M2-like macrophages induces fibro-adipogenic progenitors activation and muscle regeneration.
    Nat Commun 13(1) 7058(2022) 36411280
    Kimura K, Ramirez K, Nguyen TAV, Yamashiro Y, Sada A, Yanagisawa H.
    Contribution of PDGFRα-positive cells in maintenance and injury responses in mouse large vessels.
    Sci Rep 11(1) 8683(2021) 33883668