Strain Data Sheet

RBRC09616

Information1

Image
BRC No.RBRC09616
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Pdgfra<tm1.1(EGFP/cre/ERT2)Hyma>
Former Common namePDGFRα-GFPCreERT2
H-2 Haplotype
ES Cell lineKTPU8 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6JJcl
Appearance
Strain development
Strain description
Colony maintenance
ReferencesGenesis., 53, 329-336 (2015). 25884589

Health Report

Examination Date / Room / Rack2023/02/20Room:4-ARack:E
2023/02/20Room:4-ARack:E
2022/11/21Room:4-ARack:E
2022/08/22Room:4-ARack:E
2022/05/23Room:4-ARack:E
2022/03/07Room:4-ARack:E
2021/12/06Room:4-ARack:E
2021/10/01Room:4-ARack:E
2021/09/08Room:4-ARack:E
2021/06/07Room:4-ARack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pdgfraplatelet derived growth factor receptor, alpha polypeptide5Pdgfra<tm1.1GFP/cre/ERT2Hyma>targeted mutation 1.1, Hiroyuki Miwa

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ERT2estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)5ERT2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site5Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)5

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase5cre

Information2

Donor DNAJellyfish GFP cDNA, Phage P1 Cre recombinase cDNA, human Estrogen receptor binding domain (ERT2)cDNA, yeast FRT (flipase recombination target) site, mouse Pdgfra genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorTakumi Era (Kumamoto University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Mouse of the Month Sep 2016
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Kimura K, Ramirez K, Nguyen TAV, Yamashiro Y, Sada A, Yanagisawa H.
Contribution of PDGFRα-positive cells in maintenance and injury responses in mouse large vessels.
Sci Rep 11(1) 8683(2021) 33883668