Strain Data Sheet

RBRC09609

Strain Information

Image
BRC No.RBRC09609
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Gfra1<tm2.1Jmi>
Former Common name
H-2 Haplotype
ES Cell lineRW4 [129X1/SvJ]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Conditional ablation of GFRalpha1 in postmigratory enteric neurons triggers unconventional neuronal death in the colon and causes a Hirschsprung's disease phenotype.
Uesaka T, Jain S, Yonemura S, Uchiyama Y, Milbrandt J, Enomoto H
Development, 134: 2171-2181(2007). 17507417

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frtyeast FRT (flippase recombination target) site19Frt
GFPGreen Fluorescent Protein (Aequorea victoria)19
Gfra1glial cell line derived neurotrophic factor family receptor alpha 119Gfra1targeted mutation 2.1, Jeffrey Milbrandt
loxPphage P1 loxP19loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal cell death(MP:0000313)

  • abnormal cell morphology(MP:0000358)

  • abnormal enteric ganglia morphology(MP:0001045)

  • abnormal enteric nervous system morphology(MP:0001044)

  • abnormal nervous system development(MP:0003861)
    • more 5 phenotypes
  • abnormal neuronal precursor proliferation(MP:0004948)

  • absent enteric neurons(MP:0001048)

  • absent kidney(MP:0000520)

  • neonatal lethality, complete penetrance(MP:0011087)

  • nervous system phenotype(MP:0003631)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP site, Jellyfish GFP cDNA, yeast FRT (flipase recombination target) site, mouse Gfra1 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 134: 2171-2181(2007).
    DepositorHideki Enomoto (Kobe University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data