RBRC09607

Information1

Image
BRC No.RBRC09607
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Avp<tm1(Eluc)>Chron
Former Common nameAVP-ELuc
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Kenichi Honma and Sato Honma, Graduate School of Medicine, Hokkaido University (2015). C57BL/6J background.
Strain descriptionKnock-in mice of emerald-luciferase (Eluc) in the Arginine vasopressin (AVP) gene locus. AVP is expressed at the paraventricular hypothalamic nucleus, supraoptic nucleus and suprachiasmatic nucleus. Homozygotes die by postnadal day 10. A DNA construct of ELuc-Neo was knocked into the regions upstream of the translation initiation site of the mouse AVP locus.
Colony maintenance
ReferencesEur. J. Neurosci., 42, 2678-2689 (2015). 26342201

Health Report

Examination Date / Room / Rack2018/04/16Room:3-ARack:H
2018/02/19Room:3-ARack:H
2017/12/18Room:3-ARack:H
2017/10/16Room:3-ARack:H
2017/08/21Room:3-ARack:H
2017/06/19Room:3-ARack:H
2017/04/17Room:3-ARack:H
2017/02/20Room:3-ARack:H

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Avparginine vasopressin2Avp<tm1(Eluc)>targeted mutation, Sato Honma

Gene symbolGene nameChr.Allele symbolAllele namePromoter
ELucEmerald luc luciferase (Pyrearinus termitilluminans)2ELuc

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site2Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site2Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)2mouse Phosphoglycerate kinase promoter (PGK promoter), gb2 promoter

Information2

Donor DNAbeetle Emerald-Luciferase cDNA, mouse PEST sequence(ornithine dehydrogenase cDNA), mouse Pgk promotor, phage T7 gb2 promotor, E. coli Neomycine resistant gene, mouse Pgk PolyA, yeast FRT (flipase recombination target) sites, mouse Avp genomic DNA
Research applicationFLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Eur J Neurosci. 42:2678-2689, 2015. doi: 10.1111/ejn.13061. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Prior nortification of the use of C57BL/6J-(AVPELuc) mouse.
DepositorSato Honma (Hokkaido University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data