Strain Data Sheet

RBRC09595

Information1

Image
BRC No.RBRC09595
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Keap1<tm2Mym>
Former Common nameKeap1 flox
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesMol Cell Biol., 30, 3016-3026 (2010). 20404090
Biochem. Biophys. Res. Commun., 339, 79-88 (2006). 16293230

Health Report

Examination Date / Room / Rack2023/02/27Room:4-BRack:I
2022/11/29Room:4-BRack:I
2022/08/29Room:4-BRack:I
2022/05/31Room:4-BRack:I
2022/03/07Room:4-BRack:I
2021/12/06Room:4-BRack:I
2021/09/08Room:4-BRack:I
2021/08/16Room:3-DRack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Keap1kelch-like ECH-associated protein 19Keap1<tm2Mym>targeted mutation 2, Masayuki Yamamoto

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)9EGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP9loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP9loxP

Information2

Donor DNAPhage P1 LoxP sites, Splicing accepter, jellyfish EGFP cDNA, mouse Keap1 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem Biophys Res Commun., 339, 79-88 (2006)., Mol Cell Biol., 30, 3016-3026 (2010).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorMasayuki Yamamoto (Tohoku University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Vallelian F, Buzzi RM, Pfefferlé M, Yalamanoglu A, Dubach IL, Wassmer A, Gentinetta T, Hansen K, Humar R, Schulthess N, Schaer CA, Schaer DJ.
Heme-stress activated NRF2 skews fate trajectories of bone marrow cells from dendritic cells towards red pulp-like macrophages in hemolytic anemia.
Cell Death Differ (2022) 35031770