RBRC09594

Information1

Image
BRC No.RBRC09594
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-AfmTm(NLSD-Cre)38Card
Former Common nameAfm-Cre, 38
H-2 Haplotype
ES Cell line6NK-7 [C57BL/6N]
Background strain
Appearance
Strain development科研費基盤B『可変型遺伝子トラップクローンを利用したCre-driverマウスの作製』(平成23〜25年度)研究代表者:荒木正健(寄託者)により作製した。熊本大学と理研BRCの共同研究成果。荒木喜美(熊本大学)がC57BL/6マウス胚盤胞から樹立した6NK-7 ES細胞を使用した。キメラマウス(C57BL/6)♂とC57BL/6NJcl♀を交配して得た産仔♂の精子を凍結保存。
Strain description6NK-7 ES細胞 (C57BL/6) のAfm遺伝子のプロモーター領域の下流に、NLS-SD-intron-SA-Cre遺伝子をノックインすることで作製したCre-driverマウスライン。
Colony maintenance
References

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Afmafamin5Afm

Information2

Donor DNAmouse En2 splice acceptor, mouse PGK promoter, mouse PGK polyA signal, Streptomyces alboniger puromycin resistant gene, phage P1 cre recombinase, SV40 nuclear localization signal, yeast FRT (flipase recombination target) sites, phage P1 loxP and mutant lox sequence, E. coli pSP73 vector sequence, mouse Afm genomic sequence
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (後日指定)In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorMasatake Araki (Kumamoto University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.

BRC mice in Publications

No Data