Strain Data Sheet

RBRC09593

Strain Information

Image
BRC No.RBRC09593
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-ROSA26Tm(SAEiCE)16Card
Former Common nameR26SAEiCE, 16
H-2 Haplotype
ES Cell line6NK-7 [C57BL/6N]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
human estrogen receptor cDNA6
En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)6En2
Frtyeast FRT (flippase recombination target) site6Frt
Frtyeast FRT (flippase recombination target) site6Frt
Frtyeast FRT (flippase recombination target) site6Frt
Gt(ROSA26)Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA26)Sor
iCrephage P1 codon improved Cre (iCre)6iCre
lox2272phage P1 loxP variant6lox2272
loxPphage P1 loxP6loxP
puropuromycin acyltransferase (Streptomyces alboniger)6puro

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP and mutant lox sequence, mouse En2 splice acceptor, human estrogen receptor cDNA(ESR1), phage P1 cre recombinase, mouse PGK promoter, Streptomyces alboniger puromycin resistant gene, mouse PGK polyA signal, yeast FRT (flipase recombination target) sites, E. coli pSP73 vector sequence, mouse ROSA26 genomic sequence
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorMasatake Araki (Kumamoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data