RBRC09592

Information1

Image
BRC No.RBRC09592
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-ROSA26Tm(SAEiCE)13Card
Former Common nameR26SAEiCE, 13
H-2 Haplotype
ES Cell line6NK-7 [C57BL/6N]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / Rack2020/06/01Room:3-CRack:E
2020/02/17Room:3-CRack:E
2019/12/16Room:3-CRack:E
2019/10/21Room:3-CRack:E
2019/08/26Room:3-CRack:E
2019/07/08Room:3-CRack:E
2019/07/08Room:3-CRack:E
2019/06/17Room:3-CRack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gt(ROSA26)Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA26)Sor

Gene symbolGene nameChr.Allele symbolAllele namePromoter
ERT2estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)6ERT2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)6En2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
iCrephage P1 codon improved Cre (iCre)6iCre

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lox2272mutant lox sequence6lox2272

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lox71mutant lox sequence6lox71

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
puropuromycin resistance gene (Streptomyces alboniger)6puromouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAphage P1 loxP and mutant lox sequence, mouse En2 splice acceptor, human estrogen receptor cDNA(ESR1), phage P1 cre recombinase, mouse PGK promoter, Streptomyces alboniger puromycin resistant gene, mouse PGK polyA signal, yeast FRT (flipase recombination target) sites, E. coli pSP73 vector sequence, mouse ROSA26 genomic sequence
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (後日指定)In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorMasatake Araki (Kumamoto University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data