RBRC09585

Information1

Image
BRC No.RBRC09585
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6.Cg-Itgb7<Gt(Ayu21-T514*mERT2)2Card>
Former Common nameItgb3-mERT2, 1536
H-2 Haplotype
ES Cell lineKTPU8 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development科研費基盤B『可変型遺伝子トラップクローンを利用したCre-driverマウスの作製』(平成23〜25年度)研究代表者:荒木正健(寄託者)により作製した。熊本大学と理研BRCの共同研究成果。TT2 (B6CBAF1) ES細胞をフィーダーフリー化したKTPU8 ES細胞を用いて作製した可変型遺伝子トラップクローンを用いて作出。C57BL/6とCBAの混合背景。キメラマウス(B6とCBAのF1)♂とC57BL/6NJcl♀を交配して得たN1♂の精子を凍結保存。
Strain descriptionItgb7遺伝子をトラップした可変型遺伝子トラップクローン (Ayu21-T514) ES細胞のレポーター遺伝子をCre-mERT2遺伝子に置換することで作製したCreER-driverマウスライン。レポーターマウス (R26R) と交配し、得られた産仔にタモキシフェンを投与した後、X-gal染色を行った。耳、胸腺、肝臓、生殖組織において、部分的にCre-mERT2による組換えが検出された。
Colony maintenance
References

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Itgb7integrin beta 715Itgb7

Information2

Donor DNAmouse En2 splice acceptor, mouse PGK promoter, mouse PGK polyA signal, Streptomyces alboniger puromycin resistant gene, phage P1 cre recombinase, mouse estrogen receptor, yeast FRT (flipase recombination target) sites, phage P1 loxP and mutant lox sequence, E. coli pSP73 vector sequence, mouse Itgb7 genomic sequence
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (後日指定)In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorMasatake Araki (Kumamoto University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.

BRC mice in Publications

No Data