Strain Data Sheet

RBRC09550

Strain Information

Image
BRC No.RBRC09550
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Actb<tm2.1(tetO-YCnano50)Kftnk>
Former Common nametetO-YCnano50, B6;129-Actb<tm1(tetO-YCnano50)Kftnk>/Kftnk(tetO-YCnano50)
H-2 Haplotype
ES Cell lineG0G1
Background strainC57BL/6JJcl
Appearance
Strain development
Strain description
Colony maintenance
ReferencesCell Rep, 8(1):311-318 (2014). 24981861
Nat. Neurosci., 22(5):770-777 (2019). 30988523
Hippocampus 33296119

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Actbactin, beta5Actb<tm1(tetO-YCnano50)Kftnk>targeted mutation 1, Kenji Tanaka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site5Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
YCnano50Yellow Cameleon-Nano505YCnano50E. coli tet operator, cytomegalovirus CMV promoter

Ordering Information

Donor DNAPhage P1 loxP sites, E. coli Tetracycline operator site, Cytomegalovirus CMV minimum promoter, Rabbit Beta globin intron, human M13 peptide from MYLK, human calmodulin cDNA, jellyfish ECFP cDNA, jellyfish EYFP cDNA, SV40 polyA signal, yeast FRT (flipase recombination target) site, mouse beta actin genomic DNA
Research applicationFLP/frt system
Fluorescent Proteins/lacZ System
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Rep., 8(1), 311-318 (2014).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, RECIPIENT must inform the DEPOSITOR of his project using the Approval Form. If the use of this BIOLOGICAL RESOURCE results in scientific publications, the RECIPIENT would invite Kenji F. Tanaka to participate as co-author of publications to which he has made substantial intellectual contribution and for which he undertakes responsibilities of co-authorship.
DepositorKenji Tanaka (Keio University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Tsunematsu T, Sakata S, Sanagi T, Tanaka KF, Matsui K.
Region-Specific and State-Dependent Astrocyte Ca2+ Dynamics during the Sleep-Wake Cycle in Mice.
J Neurosci 41(25) 5440-5452(2021) 34006590