Strain Data Sheet

RBRC09545

Information1

Image
BRC No.RBRC09545
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK-Tg(Slc6a3-tTA)15Kftnk
Former Common nameDAT-tTA line15, STOCK-Tg(Slc6a3-tTA)15Kftnk(DAT-tTA line15)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) siteUNFrt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tTAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)UNtTAmouse Slc6a3 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 PolyA signalUN

Information2

Donor DNAmouse Slc6a3 genomic DNA (BAC clone RP24-158J12), E. coli tet repressor (tetR), herpes simplex virus (HSV)VP16 activation domain, SV40 polyA additional signal, P1 Phage loxP sequence, yeast FRT (flipase recombination target) site
Research applicationCre/loxP system
FLP/frt system
Tet system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research. If the use of this BIOLOGICAL RESOURCE results in scientific publications, the RECIPIENT would invite Kenji F. Tanaka to participate as co-author of publications to which he has made substantial intellectual contribution and for which he undertakes responsibilities of co-authorship.
DepositorKenji Tanaka (Keio University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data