Strain Data Sheet

RBRC09538

Information1

Image
BRC No.RBRC09538
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-Tgfbi<tm1(TGFBI)Dokm>
Former Common nameTGFBi R124H
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strainC57BL/6JJcl
Appearance
Strain development
Strain description
Colony maintenance
ReferencesPLoS One. 2015 Jul 21;10(7):e0133397. doi: 10.1371/journal.pone.0133397. eCollection 2015. 26197481

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tgfbitransforming growth factor, beta induced13Tgfbi<tm1(TGFBI)Dokm>targeted mutation 1, Department of Ophtalomology, Keio University school of Medicine

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site13Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
TGFBItransforming growth factor, beta induced (human)13TGFBI

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP13loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP13loxP

Information2

Donor DNAPhage P1 loxP sites, human TGFBI (R124H) cDNA, SV40 polyA, yeast FRT (flipase recombination target) site, mouse Tgfbi genomic DNA
Research applicationCre/loxP system
FLP/frt system
Mouse Models for Human Disease
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One. 2015 10(7):e0133397.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorShigeto Shimmura (Keio University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Mouse of the Month Mar 2016
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data