RBRC09537

Information1

Image
BRC No.RBRC09537
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-Pgk1<tm1(CAG-mcherry-NLS,PGK-Puro)Koba>
Former Common namePgk1-RED (Puro+) mouse, Momiji mouse
H-2 Haplotype
ES Cell lineEGR-101 [C57BL/6N]
Background strain
Appearance
Strain development東京医科歯科大学・小林慎先生(2011〜2013年)C57BL/6N系統。C57BL/6N由来ES細胞(EGR-101)を用いて作出。http://www.deras.biken.osaka-u.ac.jp/faq/answer01.htmlB6Nマウス(日本クレア)との交配により維持している。
Strain descriptionPgk1-eGFP(Neo+)マウスと掛け合わせることにより、X染色体不活性化、及び再活性化をモニタリングできるマウス。雄は3-5ヶ月齢で死亡する傾向傾向にある。
Colony maintenance
ReferencesDevelopment, 143(16):2958-64 (2016). 27471261
Dev. Growth Differ. (2017). 28635043

Health Report

Examination Date / Room / Rack2020/02/17Room:3-ARack:J
2019/12/16Room:3-ARack:J
2019/10/21Room:3-ARack:J
2019/08/26Room:3-ARack:J
2019/06/17Room:3-ARack:J
2019/04/15Room:3-ARack:J
2019/02/18Room:3-ARack:J
2018/12/17Room:3-ARack:J

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Pgk1phosphoglycerate kinase 1XPgk1<tm1(CAG-mcherry-NLS,PGK-Puro)Koba>targeted mutation 1, Shin Kobayashi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) siteXFrt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) siteXFrt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
MBD1methyl-CpG binding domain protein 1 (human)XMBD1

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
mCherryRed Fluorescent Protein (Discosoma sp.)XmCherryCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
puropuromycin resistance gene (Streptomyces alboniger)Xpuromouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAPhage P1 loxP sites, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Discosoma sp. mCherry cDNA, human MBD1 cDNA, Yeast yeast FRT (flippase recombination target) sites, Streptomyces alboniger puromycin-resistance gene, mouse Pgk1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 143(16):2958-64 (2016).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorShin Kobayashi (National Institute of Advanced Industrial Science and Technology)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityLive mouse (1 to 3 months)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data