Strain Data Sheet

RBRC09537

Information1

Image
BRC No.RBRC09537
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-Pgk1<tm1(CAG-mcherry-NLS,PGK-Puro)Koba>
Former Common namePgk1-RED (Puro+) mouse, Momiji mouse
H-2 Haplotype
ES Cell lineEGR-101 [C57BL/6N]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesDev. Growth Differ. (2017). 28635043
Development, 143(16):2958-64 (2016). 27471261

Health Report

Examination Date / Room / Rack2023/01/10Room:3-ARack:J
2022/10/11Room:3-ARack:J
2022/07/11Room:3-ARack:J
2022/04/11Room:3-ARack:J
2022/01/24Room:3-ARack:J
2021/10/26Room:3-ARack:J
2021/07/26Room:3-ARack:J
2021/04/26Room:3-ARack:J

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pgk1phosphoglycerate kinase 1XPgk1<tm1(CAG-mcherry-NLS,PGK-Puro)Koba>targeted mutation 1, Shin Kobayashi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) siteXFrt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) siteXFrt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
MBD1methyl-CpG binding domain protein 1 (human)XMBD1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mCherryRed Fluorescent Protein (Discosoma sp.)XmCherryCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
puropuromycin resistance gene (Streptomyces alboniger)Xpuromouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAPhage P1 loxP sites, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Discosoma sp. mCherry cDNA, human MBD1 cDNA, Yeast yeast FRT (flippase recombination target) sites, Streptomyces alboniger puromycin-resistance gene, mouse Pgk1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 143(16):2958-64 (2016).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorShin Kobayashi (National Institute of Advanced Industrial Science and Technology)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: FP license of TBUSA
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP

BRC mice in Publications

No Data