RBRC09532

Information1

Image
BRC No.RBRC09532
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Hprt<tm1(CAG-eGFP-NLS)Koba>
Former Common nameHprt-eGFP, Momiji mouse
H-2 Haplotype
ES Cell lineBPES11[carries Hprt<b-m3>]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesDev. Growth Differ. (2017). 28635043
Development, 143(16):2958-64 (2016). 27471261
Methods Mol Biol., 1861:73-89 (2018). 30218361

Health Report

Examination Date / Room / Rack2020/04/06Room:3-3Rack:D
2020/02/03Room:3-3Rack:D
2019/12/02Room:3-3Rack:D
2019/10/16Room:3-3Rack:D
2019/08/05Room:3-3Rack:D
2019/06/03Room:3-3Rack:D
2019/04/01Room:3-3Rack:D
2019/02/04Room:3-3Rack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Hprthypoxanthine guanine phosphoribosyl transferaseXHprt<tm1(CAG-eGFP-NLS)Koba>targeted mutation 1, Shin Kobayashi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
EGFPEnhanced Green Fluorescent Protein, F64L and S65T (Aequorea victoria)XEGFPCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) siteXFrt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
nlsSV40 large T antigen nuclear localization signal (NLS)Xnls

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), jellyfish GFP cDNA, NLS derived from the human MBD1 cDNA, mouse Hprt genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 143(16):2958-64 (2016).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorShin Kobayashi (National Institute of Advanced Industrial Science and Technology)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityLive mouse (1 to 3 months)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data