Strain Data Sheet

RBRC09510

Strain Information

Image
BRC No.RBRC09510
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;Cg-Rsph4a<tm1.2Hmd>
Former Common nameRsph4a(B18) flox
H-2 Haplotype
ES Cell lineG4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strainC57BL/6JJcl
Appearance
Strain development
Strain description
Colony maintenance
References
Absence of Radial Spokes in Mouse Node Cilia Is Required for Rotational Movement but Confers Ultrastructural Instability as a Trade-Off.
Shinohara K, Chen D, Nishida T, Misaki K, Yonemura S, Hamada H
Dev. Cell., 35(2):236-46 (2015). doi: 10.1016/j.devcel.2015.10.001. 26506310

Health Report

Examination Date / Room / Rack2024/05/20Room:4-ARack:B
2024/02/19Room:4-ARack:B
2023/11/20Room:4-ARack:B

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frtyeast FRT (flippase recombination target) site10Frt
  • primary ciliary dyskinesia 11(MedGEN)
    • Rsph4aradial spoke head 4 homolog A (Chlamydomonas)10Rsph4atargeted mutation 1.2, Hiroshi Hamada
  • primary ciliary dyskinesia 11(MedGEN)
    • loxPphage P1 loxP10loxP
  • primary ciliary dyskinesia 11(MedGEN)
    • loxPphage P1 loxP10loxP
  • primary ciliary dyskinesia 11(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, yeast FRT (flippase recombination target) site, mouse Rsph4a genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Cell., 35(2):236-46 (2015). doi: 10.1016/j.devcel.2015.10.001.
    DepositorHiroshi Hamada (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data