Strain Data Sheet

RBRC09508

Strain Information

Image
BRC No.RBRC09508
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(RP11-94J18/RP11-662G11)BMy
Former Common name3q21q26 mouse line B
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain development
Strain description
Colony maintenance
References
A remote GATA2 hematopoietic enhancer drives leukemogenesis in inv(3)(q21;q26) by activating EVI1 expression.
Yamazaki H, Suzuki M, Otsuki A, Shimizu R, Bresnick E H, Engel J D, Yamamoto M
Cancer Cell., 25, 415-427 (2014). 24703906

GATA2 haploinsufficiency accelerates EVI1-driven leukemogenesis.
Saori Katayama, Mikiko Suzuki, Ayaka Yamaoka, Nadine Keleku-Lukwete, Fumiki Katsuoka, Akihito Otsuki, Shigeo Kure, James Douglas Engel, Masayuki Yamamoto
Blood. 130(7):908-919 (2017). 28630119

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) siteUNFrt
Frt yeast FRT (flippase recombination target) siteUNFrt
MECOM MDS1 and EVI1 complex locusUNMECOM human GATA2 enhancer
loxP phage P1 loxPUNloxP
neo neomycin resistance gene (E. coli)UN

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal acute inflammation (MP:0002498)

  • anemia (MP:0001577)

  • enlarged spleen (MP:0000691)

  • increased erythroid progenitor cell number (MP:0003135)

  • increased leukemia incidence (MP:0002026)
    • more 2 phenotypes
  • increased leukocyte cell number (MP:0000218)

  • lethality, complete penetrance (MP:0011400)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman RP11-94J18, human RP11-662G11, phage P1 loxP site, yeast FRT (flipase recombination target) sites, E. coli neo
    Research applicationCre/loxP system
    Hematological Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cancer Cell 25, 415-427 (2014). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE,involving the DEPOSITOR in author byline is requested (by Oct 2017).
    DepositorMasayuki Yamamoto (Tohoku University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Zang W, Koike Y, Nishimura K, Tanaka A, Yamazaki H, Yamasaki T, Ito H, Zhang Y, Aoyama Y, Saika W, Xiao M, Hasegawa C, Kunimoto H, Nakajima H, Ishikawa F, Takaori-Kondo A, Inoue D.
    BRD4 and MYB inhibition overcomes venetoclax resistance in EVI1-rearranged acute myeloid leukemia.
    Sci Rep 15(1) 37099(2025) 41131284