RBRC09500

Information1

Image
BRC No.RBRC09500
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6D2-Wbp2nl<tm1b(EUCOMM)Osb>
Former Common namePAWP KO
H-2 Haplotype
ES Cell lineEGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesBiol Reprod. 2015 Oct;93(4):94. doi: 10.1095/biolreprod.115.131441. Epub 2015 Sep 16. 26377222

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Wbp2nlWBP2 N-terminal like15Wbp2nl<tm1b(EUCOMM)Osb>targeted mutation 1b, Research Institute for Microbial Diseases, Osaka University

Gene symbolGene nameChr.Allele symbolAllele namePromoter
En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)15En2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site15Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Iresinternal ribosomal entry site (EMCV)15

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)15

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP15loxP

Information2

Donor DNAEn2 splice acceptor (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E.coli LacZ, SV40 polyadenylation site, hBactP (human beta-actin promoter), yeast FRT (flipase recombination target) sites, phage P1 loxP, mouse Wbp2nl genomic DNA【クラス1】
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol Reprod. 2015 Oct;93(4):94. doi: 10.1095/biolreprod.115.131441. Epub 2015 Sep 16.Using the BIOLOGICAL RESOURCE is limited to the academic research of academic institutions. This strain was established by the DEPOSITOR by using knockout ES cells of EUCOMM Consortium. The RECIPIENT shall acknowledge the EUCOMM Consortium as the source of the ES cells of the strain.The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Lab HP

BRC mice in Publications

No Data