RBRC09499

Information1

Image
BRC No.RBRC09499
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129R1-Eif3b<Gt(GT529)Skmz>
Former Common namep116, B6.129R1-Tg(p116)
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6JJcl
Appearance
Strain development
Strain description
Colony maintenance
ReferencesJ. Biochem., 131, 833-837 (2002). 12038979

Health Report

Examination Date / Room / Rack2017/03/27Room:4-ARack:J
2017/01/30Room:4-ARack:J
2016/11/28Room:4-ARack:J
2016/09/26Room:4-ARack:J
2016/07/25Room:4-ARack:J
2016/05/30Room:4-ARack:J
2016/03/28Room:4-ARack:J

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Eif3beukaryotic translation initiation factor 3, subunit B5Eif3b<Gt(GT529)Skmz>eukaryotic translation initiation factor 3, subunit B; gene trap mutation GT529, Kazuhisa Sekimizu

Gene symbolGene nameChr.Allele symbolAllele namePromoter
En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)5En2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Iresinternal ribosomal entry site (EMCV)5

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)5

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)5

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signal5

Information2

Donor DNAmouse En2 splice acceptor, E. coli lacZ, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli neo, SV40 polyadenylation site
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biochem., 131, 833-837 (2002).
DepositorKazuhisa Sekimizu (The University of Tokyo)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data