RBRC09497

Information1

Image
BRC No.RBRC09497
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Rag2<tm1Tgi>
Former Common nameRag2-EGFP KI, Rag2:GFP, C57BL/6-Rag2<tm1EGFP>
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6JJcl
Appearance
Strain development
Strain description
Colony maintenance
ReferencesInt Immunol., 15, 393-402 (2003). 12618483

Health Report

Examination Date / Room / Rack2019/01/15Room:2-検Rack:F
2018/11/12Room:2-検Rack:F
2018/09/10Room:2-検Rack:F
2018/07/09Room:2-検Rack:F
2018/05/14Room:2-検Rack:F
2018/03/12Room:2-検Rack:F
2018/01/15Room:2-検Rack:F
2017/11/13Room:2-検Rack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Rag2recombination activating gene 22Rag2<tm1Tgi>targeted mutation 1, Masaru Taniguchi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
EGFPEnhanced Green Fluorescent Protein, F64L and S65T (Aequorea victoria)2EGFP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)2herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAPhage P1 loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neomycin resistance gene, Jellyfish EGFP cDNA, mouse Rag2 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Kondo E et al. Int Immunol 15:393-402, 2003.
DepositorMasaru Taniguchi (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data