Strain Data Sheet

RBRC09497

Strain Information

Image
BRC No.RBRC09497
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Rag2<tm1Tgi>
Former Common nameRag2-EGFP KI, Rag2:GFP, C57BL/6-Rag2<tm1EGFP>
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6JJcl
Appearance
Strain development
Strain description
Colony maintenance
References
Expression of recombination-activating gene in mature peripheral T cells in Peyer's patch.
Kondo E, Wakao H, Koseki H, Takemori T, Kojo S, Harada M, Takahashi M, Sakata S, Shimizu C, Ito T, Nakayama T, Taniguchi M
Int. Immunol., 15, 393-402 (2003). 12618483

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)2EGFP
  • combined immunodeficiency with skin granulomas(MedGEN)

  • Omenn syndrome(DisGeNET, MedGEN)

  • severe combined immunodeficiency, autosomal recessive, T cell-negative, B cell-negative, NK cell-positive(DisGeNET, MedGEN)
    • Rag2recombination activating gene 22Rag2targeted mutation 1, Masaru Taniguchi
  • combined immunodeficiency with skin granulomas(MedGEN)

  • Omenn syndrome(DisGeNET, MedGEN)

  • severe combined immunodeficiency, autosomal recessive, T cell-negative, B cell-negative, NK cell-positive(DisGeNET, MedGEN)
    • loxPphage P1 loxP2loxP
  • combined immunodeficiency with skin granulomas(MedGEN)

  • Omenn syndrome(DisGeNET, MedGEN)

  • severe combined immunodeficiency, autosomal recessive, T cell-negative, B cell-negative, NK cell-positive(DisGeNET, MedGEN)
    • loxPphage P1 loxP2loxP
  • combined immunodeficiency with skin granulomas(MedGEN)

  • Omenn syndrome(DisGeNET, MedGEN)

  • severe combined immunodeficiency, autosomal recessive, T cell-negative, B cell-negative, NK cell-positive(DisGeNET, MedGEN)
    • neoneomycin resistance gene (E. coli)2herpes simplex virus thymidine kinase promoter (HSV tk promoter)
  • combined immunodeficiency with skin granulomas(MedGEN)

  • Omenn syndrome(DisGeNET, MedGEN)

  • severe combined immunodeficiency, autosomal recessive, T cell-negative, B cell-negative, NK cell-positive(DisGeNET, MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal Peyer's patch morphology(MP:0000696)

  • absent B cells(MP:0008071)

  • absent T cells(MP:0008070)

  • arrested B cell differentiation(MP:0001802)

  • arrested T cell differentiation(MP:0001825)
    • more 3 phenotypes
  • decreased B cell number(MP:0005017)

  • decreased double-positive T cell number(MP:0005092)

  • decreased thymocyte number(MP:0000715)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neomycin resistance gene, Jellyfish EGFP cDNA, mouse Rag2 genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Int. Immunol., 15:393-402 (2003).
    DepositorMasaru Taniguchi (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Naito Y, Koyama S, Masuhiro K, Hirai T, Uenami T, Inoue T, Osa A, Machiyama H, Watanabe G, Sax N, Villa J, Kinugasa-Katayama Y, Nojima S, Yaga M, Hosono Y, Okuzaki D, Satoh S, Tsuda T, Nakanishi Y, Suga Y, Morita T, Fukushima K, Nishide M, Shiroyama T, Miyake K, Iwahori K, Hirata H, Nagatomo I, Yano Y, Tamiya M, Kumagai T, Takemoto N, Inohara H, Yamasaki S, Yamashita K, Aoshi T, Akbay EA, Hosen N, Shintani Y, Takamatsu H, Mori M, Takeda Y, Kumanogoh A.
    Tumor-derived semaphorin 4A improves PD-1-blocking antibody efficacy by enhancing CD8+ T cell cytotoxicity and proliferation.
    Sci Adv 9(20) eade0718(2023) 37205755