Strain Data Sheet

RBRC09486

Information1

Image
BRC No.RBRC09486
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Xcr1<tm1Ksho>
Former Common nameXCR1-venus, XCR1-venus B6
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Tsuneyasu Kaisho, Research Center for Allergy and Immunologuy, RIKEN around 2008.
Strain descriptionA chemokine receptor, XCR1, is a receptor for XCL1 and expressed dominantly in one dendritic cell subset. XCR1-venus mice were generated by knocking the gene encoding a fluorescence protein, venus (derived from Dr. Atsushi Miyawaki at BRI) into the murine XCR1 gene locus. In XCR1-venus mice, XCR1-expressing cells can be detected by venus expression.
Colony maintenance
ReferencesJ. Immunol., 190, 6071-6082 (2013). 23670193

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Xcr1chemokine (C motif) receptor 19Xcr1<tm1Ksho>targeted mutation 1, Tsuneyasu Kaisho

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site9Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site9Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
VenusYellow Fluorescent Protein (Aequorea victoria)9Venus

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP9loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP9loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)9herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAAequorea coerulescens mutant GFP (venus) cDNA, Bos taurus bGHpolyA, Bacteriophage P1 loxP sites, Saccharomyces cerevisiae FRT sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), Escherichia coli Neomycin resistant gene, HSV TK polyA signal, mouse Xcr1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Immunology and Inflammation Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Immunol., 190, 6071-6082 (2013). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Venus using Approval form (form V) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp).
DepositorTsuneyasu Kaisho (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Mouse of the Month Jan 2016
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Venus using Approval form (Form V (English)) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp).
  5. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data