Strain Data Sheet
RBRC09485
Information1 | |
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| Image | |
| BRC No. | RBRC09485 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.Cg-Xcr1<tm2(HBEGF/Venus)Ksho> |
| Former Common name | XCR1-DTRvenus |
| H-2 Haplotype | |
| ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
| Background strain | C57BL/6JJcl |
| Appearance | |
| Strain development | Developed by Tsuneyasu Kaisho, Research Center for Allergy and Immunologuy, RIKEN around 2008. |
| Strain description | A chemokine receptor, XCR1, is a receptor for XCL1 and expressed dominantly in one dendritic cell subset. XCR1-DTRvenus mice were generated by knocking the gene encoding a fusion protein consisting of human diphteria toxin receptor (DTR or HBEGF) and a fluorescence protein, venus (derived from Dr. Atsushi Miyawaki at BRI) into the murine XCR1 gene locus. In XCR1-DTRvenus mice, XCR1-expressing cells can be detected by venus expression and ablated transiently by the administration of diphteria toxin. |
| Colony maintenance | |
| References | J. Immunol., 190, 6071-6082 (2013). 23670193 |
Health Report | |
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| Examination Date / Room / Rack | 2023/08/21Room:4-ARack:G 2023/05/22Room:4-ARack:G 2023/03/22Room:4-ARack:G 2023/02/20Room:4-ARack:G 2022/11/21Room:4-ARack:G 2022/08/22Room:4-ARack:G 2022/05/23Room:4-ARack:G 2022/05/23Room:4-ARack:G 2022/03/07Room:4-ARack:G 2021/12/06Room:4-ARack:G |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site9Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site9Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA (Bovine)9GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HBEGFheparin-binding EGF-like growth factor (human)9HBEGF Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter VenusYellow Fluorescent Protein (Aequorea victoria)9Venus Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)9herpes simplex virus thymidine kinase promoter (HSV tk promoter) |
Information2 | |
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| Donor DNA | human HB-EGF (DTR) cDNA, Aequorea coerulescens mutant GFP (venus) cDNA, Bos taurus bGH polyA, Saccharomyces cerevisiae FRT sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), Escherichia coli Neomycin resistance gene, HSV TK polyA signal, mouse Xcr1 genomic DNA |
| Research application | FLP/frt system Fluorescent Proteins/lacZ System Immunology and Inflammation Research |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Immunol., 190, 6071-6082 (2013). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Venus using Approval form (form V) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp). |
| Depositor | Tsuneyasu Kaisho (RIKEN) |
| Strain Status |  Live mice Frozen embryos Frozen sperm |
| Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
| Additional Info. | Mouse of the Month Jan 2016 Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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