RBRC09479

Information1

Image
BRC No.RBRC09479
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameA.129X-Ryr2<tm1(CAG-KRAS<*>G12V,-lacZ)Nobs>
Former Common nameA/J-Ryr2<tm1Nobs>
H-2 Haplotype
ES Cell lineRW4 [129X1/SvJ]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesBiochem. Biophys. Res. Commun., 452(4):1067-70 (2014). 25245290
Oncogene., 25, 7673-7679 (2006). 16785989

Health Report

Examination Date / Room / Rack2019/09/30Room:4-BRack:F
2019/07/29Room:4-BRack:F
2019/05/27Room:4-BRack:F
2019/03/25Room:4-BRack:F
2019/01/28Room:4-BRack:F
2018/11/26Room:4-BRack:F
2018/09/25Room:4-BRack:F
2018/07/30Room:4-BRack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Ryr2ryanodine receptor 2, cardiac13Ryr2<tm1(CAG-KRAS*G12V,-lacZ)Nobs>targeted mutation 1, Noboru Suzuki

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHGrowth hormone polyA (Bovine)13GH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
KRASKirsten rat sarcoma viral oncogene homolog (KRAS)13KRAS

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)13

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP13loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP13loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)13mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
nlsSV40 large T antigen nuclear localization signal (NLS)13nls

Gene symbolGene nameChr.Allele symbolAllele namePromoter
puropuromycin resistance gene (Streptomyces alboniger)13puroCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA13

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP sites, yeast HIS3 DNA, Streptomyces alboniger puromycin resistant gene, human K-rasG12V cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), SV40 nuclear localizing signal, E. coli lacZ, bovine growth hormone polyadenylation signal, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, SV40 poly A additional signal, mouse Ryr2 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorNoboru Suzuki (Mie University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data