RBRC09462

Information1

Image
BRC No.RBRC09462
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Tc (CYP3A-MAC)-Cyp3a
Former Common nameC3M/BW, STOCK Tc (CYP3A-MAC)-Cyp3a
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesACS Synth Biol., 3(12):903-914(2014). 23654256
Biochem Biophys Res Commun., 442(1-2), 44-50 (2013). 24216103
Hum Mol Genet., 22(3), 578-592 (2013). 23125282

Health Report

Examination Date / Room / Rack2019/10/16Room:3-6Rack:A
2019/08/05Room:3-6Rack:A
2019/06/03Room:3-6Rack:A
2019/04/01Room:3-6Rack:A
2019/02/04Room:3-6Rack:A
2018/12/03Room:3-6Rack:A
2018/10/01Room:3-6Rack:A
2018/08/06Room:3-6Rack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Cyp3a13cytochrome P450, family 3, subfamily a, polypeptide 135Cyp3a13<tm1Mtos>targeted mutation 1, Mitsuo Oshimura

Gene symbolGene nameChr.Allele symbolAllele namePromoter
CYP3AUNCYP3A

Gene symbolGene nameChr.Allele symbolAllele namePromoter
EGFPEnhanced Green Fluorescent Protein, F64L and S65T (Aequorea victoria)EGFPCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
hyghygromycin phasphotransferase (E. coli)hygmouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAhuman CYP3A cluster genomic DNA, Bovine growth hormone (BGH) polyA signal, chicken beta-globin insulator, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), SV40 large antigen,SV40 early promoter, SV40 Origin (Ori), polyA signal, P1 Phage loxP site, E. coli ampicilin resistance gene, E. coli Kan resistance gene, E. coli hyg resistance gene, jellyfish GFP cDNA, mouse PGK promoter, mouse chromosome 11 centromere region
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT must inform the DEPOSITOR whether its research plan includes breeding, crossing with other strains and/or modifications of an inserted DNA. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for an academic research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorHiroyuki Kugoh (Tottori University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data