Strain Data Sheet

RBRC09450

Information1

Image
BRC No.RBRC09450
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-Gt(Rosa)26Sor<tm1(CAG-GCaMP6,-mCherry)Shi>
Former Common nameRCL-G6-2A-mCh
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strainC57BL/6JJcl
Appearance
Strain development
Strain description
Colony maintenance
ReferencesPLoS One, 7, e51286, (2012). 23240011

Health Report

Examination Date / Room / Rack2022/12/12Room:3-2Rack:A
2022/09/12Room:3-2Rack:A
2022/06/13Room:3-2Rack:A
2022/03/14Room:3-2Rack:A
2021/12/14Room:3-2Rack:A
2021/09/13Room:3-2Rack:A
2021/06/14Room:3-2Rack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(CAG-GCaMP6,-mCherry)Shi>

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
F2Afoot-and-mouth disease virus (FMD) 2A peptide6F2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GCaMP6RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA6GCaMP6CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)6WPRE

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mCherryRed Fluorescent Protein (Discosoma sp.)6mCherry

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
poly Apolyadenylic acid additional signal6poly A

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Bacteriophage P1 LoxP sites, E. coli Neomycin resistance gene, GCaMP6:RSET tag, chicken smooth muscle M13, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, Rat calmodulin (CaM) cDNA(aa.2-148), Foot-and-mouth disease virus (FMDV derived) 2A peptide (F2A), Discosoma sp. mCherry cDNA, Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element(WPRE), polyA, mouse Gt(Rosa)26Sor genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and Conditions1) Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
2) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
3) The BIOLOGICAL RESOURCE must not be provided to any third party without DEPOSITOR’s permission.
4) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One. 2012 Dec 11; 7(12), e51286, 2012.
DepositorShionogi & Co., Ltd. (Shionogi & Co., Ltd)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: FP license of TBUSA
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)
  6. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data