RBRC09435

Information1

Image
BRC No.RBRC09435
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-Gt(ROSA)26Sor<tm1(Stop-3xFlag ESET,EGFP)>
Former Common nameRosa26-3xFlag-ESET-IRES-GFP- KI
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / Rack2016/10/18Room:3-CRack:A
2016/10/17Room:3-CRack:A
2016/08/22Room:3-CRack:A
2016/06/20Room:3-CRack:A
2016/04/18Room:3-CRack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(Stop-3xFlag ESET,EGFP)>

Gene symbolGene nameChr.Allele symbolAllele namePromoter
EGFPEnhanced Green Fluorescent Protein, F64L and S65T (Aequorea victoria)6EGFP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
ESET6ESET

Gene symbolGene nameChr.Allele symbolAllele namePromoter
FLAGFLAG tag (synthetic)6FLAG

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)6herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNABacteriophage loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, Stop (synthetic), FLAG tag (synthetic), mouse ESET cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Aequorea victoria EGFP cDNA, mouse Gt(ROSA)26Sor genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorAtsushi Iwama (Chiba University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data