RBRC09400

Information1

Image
BRC No.RBRC09400
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Tg(Gata1-GFP)357Mym
Former Common nameG1BAC-GFP Tg mouse line 357
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Masayuki Yamamoto, Faculty of Medicine, University of Tsukuba in 2003. A BAC DNA construct was injected into fertilized eggs from BDF1 background. Mice were maintained in a mixed background of BDF1 and ICR.
Strain descriptionBAC transgenic mice in which GFP is under the control of the Gata1 gene promoter.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesMol Cell Biol., 29(5), 1163-1175 (2009). 19103751

Health Report

Examination Date / Room / Rack2020/03/30Room:4-ARack:L
2020/01/27Room:4-ARack:L
2019/11/25Room:4-ARack:L
2019/09/30Room:4-ARack:L
2019/07/29Room:4-ARack:L
2019/05/27Room:4-ARack:L
2019/03/25Room:4-ARack:L
2019/01/28Room:4-ARack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) siteUNFrt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)UNmouse Gata1 promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPUNloxP

Information2

Donor DNAmouse Gata1 genomic DNA (BAC clone RP23-443E19), Jellyfish GFP cDNA, yeast FRT (flipase recombination target) site, Phage P1 loxP site derived from pBACe3.6 vector
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell Biol., 29, 1163-1175 (2009).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publiching the research results to be obtained by use of the BOLOGOCAL RESOURCE, involving the DEPOSITOR in author byline is requested (by May 2018).
DepositorMasayuki Yamamoto (Tohoku University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen spermFrozen sperm
Strain AvailabilityLive mouse (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data