Strain Data Sheet

RBRC09400

update : 2024-12-20

Strain Information
Image
BRC No.	RBRC09400
Type	Transgene
Species	Mus musculus
Strain name	STOCK Tg(Gata1-GFP)357Mym
Former Common name	G1BAC-GFP Tg mouse line 357
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development	Developed by Masayuki Yamamoto, Faculty of Medicine, University of Tsukuba in 2003. A BAC DNA construct was injected into fertilized eggs from BDF1 background. Mice were maintained in a mixed background of BDF1 and ICR.
Strain description	BAC transgenic mice in which GFP is under the control of the Gata1 gene promoter.
Colony maintenance	Heterozygote x Wild-type [C57BL/6JJcl]
References	Differential contribution of the Gata1 gene hematopoietic enhancer to erythroid differentiation. Suzuki M, Moriguchi T, Ohneda K, Yamamoto M Mol. Cell Biol., 29(5), 1163-1175 (2009). 19103751

Health Report
Examination Date / Room / Rack

Gene
Gene Symbol	Gene Name	Chr.	Allele Symbol	Allele Name	Common Names	Promoter	Diseases Related to This Gene
Frt	yeast FRT (flippase recombination target) site	UN	Frt				beta-thalassemia-X-linked thrombocytopenia syndrome(MedGEN) Down syndrome(MedGEN) hemolytic anemia due to erythrocyte adenosine deaminase overproduction(MedGEN) more 3 Diseases thrombocytopenia(DisGeNET) thrombocytopenia, X-linked, with or without dyserythropoietic anemia(MedGEN) X-linked dyserythropoetic anemia with abnormal platelets and neutropenia(MedGEN)
GFP	Green Fluorescent Protein (Aequorea victoria)	UN				mouse Gata1 promoter	beta-thalassemia-X-linked thrombocytopenia syndrome(MedGEN) Down syndrome(MedGEN) hemolytic anemia due to erythrocyte adenosine deaminase overproduction(MedGEN) more 3 Diseases thrombocytopenia(DisGeNET) thrombocytopenia, X-linked, with or without dyserythropoietic anemia(MedGEN) X-linked dyserythropoetic anemia with abnormal platelets and neutropenia(MedGEN)
loxP	phage P1 loxP	UN	loxP				beta-thalassemia-X-linked thrombocytopenia syndrome(MedGEN) Down syndrome(MedGEN) hemolytic anemia due to erythrocyte adenosine deaminase overproduction(MedGEN) more 3 Diseases thrombocytopenia(DisGeNET) thrombocytopenia, X-linked, with or without dyserythropoietic anemia(MedGEN) X-linked dyserythropoetic anemia with abnormal platelets and neutropenia(MedGEN)

Phenotype
Annotation by Mammalian phenotyhpe ontology	normal phenotype(MP:0002873)
Detailed phenotype data

Ordering Information
Donor DNA	mouse Gata1 genomic DNA (BAC clone RP23-443E19), Jellyfish GFP cDNA, yeast FRT (flipase recombination target) site, Phage P1 loxP site derived from pBACe3.6 vector
Research application	Fluorescent Proteins/lacZ System
Specific Term and Conditions	Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell Biol., 29(5), 1163-1175 (2009). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publiching the research results to be obtained by use of the BOLOGOCAL RESOURCE, involving the DEPOSITOR in author byline is requested (by May 2018).
Depositor	Masayuki Yamamoto (Tohoku University)
Strain Status	Frozen sperm
Strain Availability	Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month)
Additional Info.	Necessary documents for ordering: Approval form (Japanese / English) Order form (Japanese / English) Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English) Acceptance of responsibility for living modified organism (Japanese / English) Genotyping protocol -PCR-

BRC mice in Publications

No Data