RBRC09365

Information1

Image
BRC No.RBRC09365
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameSTOCK Gt(ROSA)26Sor<tm5(ACTB-tTA)Luo>Igs7<tm92.1(tetO-YCX2.60)Hze>/J
Former Common nameROSA26-ZtTA;Ai92(TITL-YCX2.60)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesPLoS One., 5(7):e11503 (2010). 20634890
Neuron., 85(5): 942-958 (2015). 25741722

Health Report

Examination Date / Room / Rack2020/02/03Room:3-3Rack:C
2019/12/02Room:3-3Rack:C
2019/10/16Room:3-3Rack:C
2019/08/05Room:3-3Rack:C
2019/07/22Room:3-3Rack:C
2019/07/22Room:3-3Rack:C
2019/06/03Room:3-3Rack:C
2019/04/01Room:3-3Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm5(ACTB-tTA)Luo>targeted mutation 5, Liqun Luo

Gene symbolGene nameChr.Allele symbolAllele namePromoter
b-geolacZ reporter gene and neomycin resistance gene6b-geo

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
tTAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)6tTA

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA6

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Igs7intergenic site 79Igs7<tm92.1(tetO-YCX2.60)Hze>targeted mutation 92.1, Hongkui Zeng

Gene symbolGene nameChr.Allele symbolAllele namePromoter
F3yeast FRT (flippase recombination target) site (synthetic) variant9F3

Gene symbolGene nameChr.Allele symbolAllele namePromoter
F5yeast FRT (flippase recombination target) site (synthetic) variant9F5

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHGrowth hormone polyA (Bovine)9GH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHGrowth hormone polyA (Bovine)9GH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Hyghygromycin phasphotransferase (E. coli)9Hygmouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)9WPRE

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP9loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP9loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signal9

Information2

Donor DNACytomegalovirus CMV enhancer, chicken β-actin promoter, P1 phage loxP sites, E. coli β-galactosidase, E. coli neomycin-resistance gene, Tet transactivator (E. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domain), SV40 polyA,/ yeast FRT (flipase recombination target) sites (FRT3 and FRT5), chicken β-globin HS4 insulater, E. coli tetO promoter, synthetic polyA, human hGH polyA, mouse PGK polyA, jellyfish CFP/YFP cDNA, Xenopus CaM, Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), bovine bGH polyA, E. coli AttB, λphage AttP, mouse PGK promoter, E. coli hygromycin resistance gene
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Tet system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuron., 85(5): 942-958 (2015).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT agrees to acknowledge PROVIDER in all oral presentations and written publications as related to the MATERIAL and provide a copy of the publication to the DEPOSITOR.
DepositorAtsushi Miyawaki (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Miyawaki Lab HP

BRC mice in Publications

No Data