Strain Data Sheet
RBRC09365
Information1 | |
|---|---|
| Image | |
| BRC No. | RBRC09365 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | STOCK Gt(ROSA)26Sor<tm5(ACTB-tTA)Luo>Igs7<tm92.1(tetO-YCX2.60)Hze>/J |
| Former Common name | ROSA26-ZtTA;Ai92(TITL-YCX2.60) |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | |
| Strain description | |
| Colony maintenance | |
| References | PLoS One., 5(7):e11503 (2010). 20634890 Neuron., 85(5): 942-958 (2015). 25741722 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | 2022/11/07Room:2-検Rack:C |
Gene | |
|---|---|
| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm5(ACTB-tTA)Luo>targeted mutation 5, Liqun Luo Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter b-geolacZ reporter gene and neomycin resistance gene6b-geo Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter tTAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)6tTA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA6 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter F3yeast FRT (flippase recombination target) site (synthetic) variant9F3 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter F5yeast FRT (flippase recombination target) site (synthetic) variant9F5 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA (Bovine)9GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA (Bovine)9GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Hyghygromycin phasphotransferase (E. coli)9Hygmouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)9WPRE Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter YCX2.60chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated ECFP (cpECFP) cDNA, rat calmodulin (CaM) cDNA, circularly permutated Venus enhanced yellow fluorescent protein cDNA9YCX2.60 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP9loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP9loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signal9 |
Information2 | |
|---|---|
| Donor DNA | Cytomegalovirus CMV enhancer, chicken β-actin promoter, P1 phage loxP sites, E. coli β-galactosidase, E. coli neomycin-resistance gene, Tet transactivator (E. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domain), SV40 polyA,/ yeast FRT (flipase recombination target) sites (FRT3 and FRT5), chicken β-globin HS4 insulater, E. coli tetO promoter, synthetic polyA, human hGH polyA, mouse PGK polyA, jellyfish CFP/YFP cDNA, Xenopus CaM, Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), bovine bGH polyA, E. coli AttB, λphage AttP, mouse PGK promoter, E. coli hygromycin resistance gene |
| Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System Tet system |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuron., 85(5): 942-958 (2015).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT agrees to acknowledge PROVIDER in all oral presentations and written publications as related to the MATERIAL and provide a copy of the publication to the DEPOSITOR. |
| Depositor | Atsushi Miyawaki (RIKEN) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Genotyping protocol -PCR- Necessary documents for ordering:
Sequence of YCX2.60 |
BRC mice in Publications |
|---|
| No Data |